Figure 2.

Rpb4-bound mRNAs correlates with Puf3-interactors. (A) Venn diagrams showing the overlapping found between the mRNAs bound by Rpb4 and Puf3 identified by RIP-Seq [44,54]. (B) Venn diagrams showing the coincidence between the Rpb4-RIP (grey circles) and Puf3-bound mRNAs (blue circles) identified by HITS-CLIP [55], RIP-CHIP [56] and PAR-CLIP [57]. (C) Venn diagrams depicting the common mRNAs bound by Rpb4 and Puf3 (for Puf3, they were selected as those in common in at least 3 of the 5 datasets indicated in B). (D) Boxplots showing the distribution of significant Rpb4 enrichment (Log2) over the two different categories of polyadenylation isoforms belonging to the genes with a particular predicted RBP binding motif (with motif or without motif). In the boxplots, the polyadenylation isoforms with no predicted motif are depicted in blue, and the isoforms with a predicted motif are denoted in red. The RBP motifs were taken from [58]. p-value <0.1 was considered significant