Figure 4.

Rpb4 physically and genetically interacts with Puf3. (A). Whole-cell extract and TAP pulldown of the different RBPs TAP-tagged strains grown in YPD medium at 30 ºC. The resulting pulldowns were analysed by western blot with antibodies against Rpb4 and TAP-tag. No tag strain was used as a negative control and the Imd2-TAP strain was employed as a control of non-specific binding. The Rpb3-TAP strain was tested as a positive control for Rpb4 binding. (B) Growth assay of the wild-type and puf3Δ mutant strains in combination with RPB4 deletion in YPD medium at the indicated temperatures. All the experiments were performed at least in duplicate with independent biological replicates