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. 2020 Oct 13;18(8):1124–1135. doi: 10.1080/15476286.2020.1829366

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — DDX55 is present in the nucleoplasm and associates with late pre-LSU complexes. (A) The sub-cellular localization of Flag-tagged DDX55 was determined by immunofluorescence (DDX55) using antibodies against the Flag tag. Co-immunofluorescence using an α-UTP14A served as a nucleolar marker and nuclear material was visualized using DAPI staining. Scale bar represents 10 μm. (B) Whole cell extracts prepared from HEK293 cells expressing DDX55-Flag were separated by sucrose density gradient centrifugation. During fractionation, a profile of absorbance at 260 nm was generated and the peaks corresponding to free proteins/small complexes, 80S mature/90S pre-ribosomes, and (pre-)40S and (pre-)60 particles are indicated (upper panel). Proteins in each fraction were separated by SDS-PAGE and analysed by western blotting using an α-Flag antibody (lower panel)