Figure 4.

In vivo functional validation. ELISA-determined anti-B₂T peptide responses of mice vaccinated with B₂T@Montanide™ (red circle, n=4), B₂T@DMSNs-156nm (green down triangle, n=6), DMSNs-156nm (blue squares, n=4) from sera collected at days 14, 21 (pre-boost) and 40 (post-boost) post-immunization. Each point depicts mean antibody titers (calculated as described in Materials and methods) ± SD for each group. No individual spontaneous reactivity was observed in the titers determined at day 0. (cf. §SI-3; Table SI-1 , and Figures SI-14, SI-15 , and SI-16 ).