KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-FKBP5 (1:500) | Santa Cruz | Cat#sc-11518; RRID: AB_2246889 |
| Rabbit anti-GR (1:1000) | Santa Cruz | Cat#sc-1004; RRID: AB_2155786 |
| Mouse anti-MR (1:100) | Millipore-Sigma | Cat#MABS496; RRID: AB_2811270 |
| Donkey anti-Rabbit 647 (1:1000) | Abcam | Cat#ab150075; RRID: AB_2752244 |
| Goat-anti-Mouse 594 (1:1000) | Abcam | Cat#ab150116; RRID: AB_2650601 |
| Donkey anti-Goat 488 (1:1000) | Invitrogen | Cat#A-11055; RRID: AB_2534102 |
| Rabbit anti-FKBP5 (1:1000) | Bethyl | Cat#A301-430A; RRID: AB_961006 |
| Goat anti-MR (1:800) | Santa Cruz | Cat# sc-6860; RRID: AB_2298883 |
| Rabbit anti-GR (1:800) | Cell Signaling | Cat#12041; RRID: AB_2631286 |
| Mouse anit-FLAG (1:5000) | Sigma | Cat#F3165; RRID: AB_259529 |
| Rat anti-Ha (1:8000) | Roche | Cat#11867423001; RRID: AB_390918 |
| Goat anti-Actin (1:5000) | Santa Cruz | Cat#sc-1616; RRID: AB_630836 |
| Chemicals, peptides, and recombinant proteins | ||
| Mm-Nr3c1-C1 | ACDBio | Cat#475261 |
| Mm-Fkbp5-C2 | ACDBio | Cat#457241-C2 |
| Mm-Nr3c2-C3 | ACDBio | Cat#456331-C3 |
| Dexamethasone | Sigma | Cat#D1159 |
| 4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate | Steraloids | Cat#Q1562-000 |
| RU486 | Sigma-Aldrich | Cat#475838 |
| Spironolactone | Sigma-Aldrich | Cat#S3378 |
| POWRUP SYBR Green Master Mix | Thermo Scientific | Cat#4368706 |
| RIPA buffer | Merck | Cat#20-188 |
| Protease Inhibitor cocktail | Sigma | Cat#04693132001 |
| Dynabeads M-280 | Thermo Scientific | Cat#11205D |
| Protein G Dynabeads | Thermo Scientific | Cat#10007D |
| Critical commercial assays | ||
| RNAscope kit | ACDBio | Cat#320850 |
| Corticosterone Double Antibody RIA kit | MP Biomedicals | Cat#0712010-CF |
| Quick-RNA Miniprep kit | Zymo Research | Cat#R0154 |
| Superscript IV kit | Thermo Scientific | Cat#18091200 |
| Experimental models: Cell lines | ||
| Primary hippocampal neurons | This paper | N/A |
| Experimental models: Organisms/strains | ||
| C57BL/6J male mice | Jackson Laboratory | Cat#000664 |
| GRNex-CKO male mice | Hartmann et al., 2017 | N/A |
| MRCamk2α-CKO male mice | Berger et al., 2006 | N/A |
| MRAmigo2-CKO male mice | McCann et al., 2021 | N/A |
| Oligonucleotides | ||
| Fkbp5-fwd 5′ CGGCGAC AGGTCTTCTACTT 3′ | Life Technologies | N/A |
| Fkbp5-rev 5′ TCTTCACCC TGCTCAGTCAT 3′ | Life Technologies | N/A |
| Nr3c1-fwd 5′ TGCTGTT TATCTCCACTGAATTACA 3′ | Life Technologies | N/A |
| Nr3c1-rev 5′ TCCTTAGGA ACTGAGGAGAGAAGC 3′ | Life Technologies | N/A |
| Nr3c2-fwd 5′ ATGGGTACC CGGTCCTAGAG 3′ | Life Technologies | N/A |
| Nr3c2-rev 5′ AAGCCTCATCT CCACACACC 3′ | Life Technologies | N/A |
| Gapdh-fwd 5′ TATGACT CCACTCACGGCAA 3′ | Life Technologies | N/A |
| Gapdh-rev 5′ ACATACTC AGCACCGGCCT 3′ | Life Technologies | N/A |
| Biotinylated-oligonucleotide probe 5′ GACTTGGTGAGAGAAAAACAG TCCCTAAGAATGGCGCCAAGCAT AAATATCTGTTGAATCAAAAATCAAG 3′ | IDT | N/A |
| Nr3c2-siRNA sequence: 5′ GTGAAGT GGGCCAAGGTACTTCCAGGAT TTAAAAACTTGCC 3′ | IDT | N/A |
| Deposited data | ||
| Human postmortem microarray data | Hawrylycz et al., 2012 | https://human.brain-map.org/static/download |
| Single-cell RNA sequencing | Saunders et al., 2018 | http://dropviz.org |
| Software and Algorithms | ||
| Seurat | Stuart et al., 2019 | https://satijalab.org/seurat/ |
| ImageJ | NIH | https://imagej.nih.gov/ij |
| Prism 7 | GraphPad | https://www.graphpad.com |