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. 2021 Jun 30;41(26):5734–5746. doi: 10.1523/JNEUROSCI.3210-20.2021

Figure 7.

Figure 7.

Bar maintains Lor-induced c-Fos immunoreactivity in POMCARH neurons. A, Experimental scheme. B, D, c-fos immunoreactivity in POMCARH neurons in Veh + Veh, Veh + Lor, Bar + Veh, and Bar + Lor on day 1 (B) and day 20 (D) after injection of Lor (9 mg/kg) for 1 h. Arrowheads point to double-labeled neurons. C, E, Quantification of c-fos immunoreactivity in POMCARH neurons in various groups on day 1 (C) and day 20 (E) after injection of Lor. Two-way ANOVA, day 1: Lor × Bar: F(1 8) = 20.11, p = 0.002; day 20: Lor × Bar: F(1 9) = 7.04, p = 0.03, with post hoc Sidak's multiple comparisons test; **p < 0.01, ***p < 0.001. $$p < 0.01 in two tailed unpaired t tests, which was not detected by the two-way ANOVA analysis. N = 3 or 4 mice per group. Data are presented as mean ± SEM with individual data points. The right panels in B, D are higher magnification images. ns, not significant.