. 2021 Jun 30;41(26):5595–5619. doi: 10.1523/JNEUROSCI.1668-20.2021

Table 1.

Statistical analysis and the number of animals/samples used in the experiments

Figure	Pre hoc	Post hoc	N (number of samples/animals per group)
2F	(1) One-way ANOVA: F_(3,14) = 11.99, p = 0.0004* (2) Two-way ANOVA: Time × Contra/Ipsi: F_(2,10) = 2.061, p = 0.1781 Time: F_(2,10) = 9.103, p = 0.0056 Contra/Ipsi: F_(1,10) = 37.02, p = 0.0001***	Bonferroni multiple comparisons test: Baseline vs 7 d: p = 0.2581 Baseline vs 28 d: p = 0.0007* Baseline vs 56 d: p = 0.0008* Bonferroni multiple comparisons test: 7 d, Contra vs Ipsi: p = 0.1509 28 d, Contra vs Ipsi: p = 0.0017** 56 d, Contra vs Ipsi: p = 0.0188*	Baseline: 5 7 d: 5 28 d: 5 56 d: 3
2G	(1) One-way ANOVA: F_(3,11) = 5.342, p = 0.0163* (2) Two-way ANOVA: Time × Contra/Ipsi: F_(2,8) = 2.576, p = 0.1369 Time: F_(2,8) = 4.606, p = 0.0467* Contra/Ipsi: F_(1,8) = 3.755, p = 0.0887	Bonferroni multiple comparisons test: Baseline vs 7 d: p > 0.9999 Baseline vs 28 d: p > 0.9999 Baseline vs 56 d: p = 0.0110*	Baseline: 4 7 d: 4 28 d: 3 56 d: 4
2L	(1) One-way ANOVA: F_(3,11) = 11.32, p = 0.0011 (2) Two-way ANOVA: Time × Contra/Ipsi: F_(2,8) = 2.696, p = 0.1273 Time: F_(2,8) = 19.27, p = 0.0009* Contra/Ipsi: F_(1,8) = 0.5483, p = 0.4802	Bonferroni multiple comparisons test: Baseline vs 7 d: p = 0.7900 Baseline vs 28 d: p = 0.3887 Baseline vs 56 d: p = 0.0045**	Baseline: 4 7 d: 4 28 d: 3 56 d: 4
2M	(1) One-way ANOVA: F_(3,11) = 1.245, p = 0.3403 (2) Two-way ANOVA: Time × Contra/Ipsi: F_(2,8) = 1.339, p = 0.3150 Time: F_(2,8) = 0.1046, p = 0.9019 Contra/Ipsi: F_(1,8) = 0.5796, p = 0.4683		Baseline: 4 7 d: 4 28 d: 3 56 d: 4
3A	Two-way ANOVA: Sex × Contra/Ipsi: F_(1,8) = 1.076, p = 0.3299 Sex: F_(1,8) = 3.669, p = 0.0918 Contra/Ipsi: F_(1,8) = 62.42, p < 0.0001****	Bonferroni multiple comparisons test: Male, Contra vs Ipsi: p = 0.0005* Female, Contra vs Ipsi: p = 0.0025	5 for each group
3B	Two-way ANOVA: Sex × Contra/Ipsi: F_(1,8) = 0.5934, p = 0.4632 Sex: F_(1,8) = 0.4058, p = 0.5419 Contra/Ipsi: F_(1,8) = 8.679, p = 0.0185*	Bonferroni multiple comparisons test: Male, Contra vs Ipsi: p = 0.0606 Female, Contra vs Ipsi: p = 0.3250	5 for each group
3C	Two-way ANOVA: Sex × Contra/Ipsi: F_(1,8) = 0.5263, p = 0.4888 Sex: F_(1,8) = 1.269, p = 0.2926 Contra/Ipsi: F_(1,8) = 0.6922, p = 0.4296		5 for each group
3D	Two-way ANOVA: Sex × Injury: F_(1,16) = 3.458, p = 0.0814 Sex: F_(1,16) = 4.536, p = 0.0491* Injury: F_(1,16) = 60.00, p < 0.0001****	Bonferroni multiple comparisons test: Male, naive vs 56 d: p < 0.0001** Female, naive vs 56 d: p = 0.0029 naive, Male vs Female: p = 0.0492* 56 d, Male vs Female: p > 0.9999	5 for each group
3E	Two-way ANOVA: Sex × Injury: F_(1,16) = 0.4679, p = 0.5038 Sex: F_(1,16) = 1.147, p = 0.3000 Injury: F_(1,16) = 3.310, p = 0.0876		5 for each group
3F	Two-way ANOVA: Sex × Injury: F_(1,16) = 0.1846, p = 0.6732 Sex: F_(1,16) = 0.06028, p = 0.8092 Injury: F_(1,16) = 0.04463, p = 0.8354		5 for each group
4I	Unpaired two-tailed t test: t₍₁₆₎ = 0.9198, p = 0.3713		K14^C^re⁺;Atoh1^fl^/^fl;TrkC^tdtomato: 9 K14^C^re^-;Atoh1^fl^/^fl;TrkC^tdtomato: 9
4J	Unpaired two-tailed t test: t₍₁₆₎ = 0.4229, p = 0.6780		K14^C^re⁺;Atoh1^fl^/^fl;TrkC^tdtomato: 9 K14^C^re^-;Atoh1^fl^/^fl;TrkC^tdtomato: 9
5E	Unpaired two-tailed t test: NF200: t₍₁₀₎ = 0.8918, p = 0.3934 CGRP: t₍₁₀₎ = 0.07865, p = 0.9389 IB4: t₍₁₀₎ = 0.03361, p = 0.9738		K14^C^re⁺;Atoh1^fl^/^fl;TrkC^tdtomato: 6 K14^C^re^-;Atoh1^fl^/^fl;TrkC^tdtomato: 6
5F	Unpaired two-tailed t test: t₍₁₀₎ = 0.6823, p = 0.5105		K14^C^re⁺;Atoh1^fl^/^fl;TrkC^tdtomato: 6 K14^C^re^-;Atoh1^fl^/^fl;TrkC^tdtomato: 6
5G	Unpaired two-tailed t test: t₍₁₂₎ = 0.7881, p = 0.4460		K14^C^re⁺;Atoh1^fl^/^fl;Npy2R^tdtomato: 8 K14^C^re^-;Atoh1^fl^/^fl;Npy2R^tdtomato: 6
6A	Two-way ANOVA: Force × Genotype: F_(6,114) = 2.065, p = 0.0627 Force: F_{(3.101,58.92)} = 61.53, p < 0.0001** Genotype: F_(1,19) = 15.20, p = 0.0010*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.8405 0.04 g: p = 0.0088** 0.07 g: p = 0.1322 0.16 g: p = 0.0409* 0.4 g: p = 0.1005 0.6 g: p = 0.1503 1 g: p = 0.1689	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
6B	Two-way ANOVA: Force × Genotype: F_(6,114) = 1.536, p = 0.1726 Force: F_{(2.339,44.44)} = 18.85, p < 0.0001** Genotype: F_(1,19) = 8.225, p = 0.0098	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.1577 0.04 g: p = 0.1566 0.07 g: p = 0.0803 0.16 g: p = 0.1203 0.4 g: p = 0.3648 0.6 g: p = 0.3138 1 g: p = 0.6102	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
6C	Two-way ANOVA: Time × Genotype: F_(5,95) = 2.753, p = 0.0229* Time: F_{(3.544,67.33)} = 14.26, p < 0.0001** Genotype: F_(1,19) = 12.50, p = 0.0022	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.7205 3 d: p = 0.0121* 7 d: p = 0.1798 14 d: p = 0.0064** 21 d: p = 0.4730 28 d: p = 0.1351	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
6D	Two-way ANOVA: Force × Genotype: F_(6,120) = 3.545, p = 0.0029 Force: F_{(3.175,63.50)} = 118.4, p < 0.0001** Genotype: F_(1,20) = 2.407, p = 0.1365		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
6E	Two-way ANOVA: Force × Genotype: F_(6,120) = 4.346, p = 0.0005* Force: F_{(1.998,39.96)} = 29.07, p < 0.0001** Genotype: F_(1,20) = 4.197, p = 0.0538		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
6F	Two-way ANOVA: Time × Genotype: F_(5,100) = 1.933, p = 0.0954 Time: F_{(3.868,77.35)} = 19.22, p < 0.0001** Genotype: F_(1,20) = 9.820, p = 0.0052	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.6235 3 d: p = 0.0582 7 d: p = 0.2048 14 d: p = 0.1786 21 d: p = 0.0666 28 d: p = 0.0845	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
6G	Two-way ANOVA: Force × Genotype: F_(7,154) = 0.9838, p = 0.4452 Force: F_{(3.754,82.58)} = 97.02, p < 0.0001**** Genotype: F_(1,22) = 0.7751, p = 0.3882		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
6H	Two-way ANOVA: Force × Genotype: F_(7,154) = 6.136, p < 0.0001** Force: F_{(1.569,34.53)} = 33.85, p < 0.0001** Genotype: F_(1,22) = 6.292, p = 0.0200*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.0500* 0.04 g: p = 0.2286 0.07 g: p = 0.9357 0.16 g: p > 0.9999	K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
6I	Two-way ANOVA: Time × Genotype: F_(5,110) = 1.413, p = 0.2252 Time: F_{(3.026,66.58)} = 70.38, p < 0.0001** Genotype: F_(1,22) = 11.02, p = 0.0031	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.3000 3 d: p = 0.0507 7 d: p = 0.1668 14 d: p = 0.0867 21 d: p = 0.0175* 28 d: p = 0.0375*	K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
6J	Two-way ANOVA: Force × Genotype: F_(6,114) = 3.021, p = 0.0089 Force: F_{(4.031,76.59)} = 72.52, p < 0.0001** Genotype: F_(1,19) = 6.657, p = 0.0183*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p > 0.9999 0.04 g: p > 0.9999 0.07 g: p = 0.1098 0.16 g: p = 0.1903 0.4 g: p = 0.1224 0.6 g: p > 0.9999 1 g: p > 0.9999	K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 11
6K	Two-way ANOVA: Force × Genotype: F_(6,114) = 4.483, p = 0.0004* Force: F_{(2.729,51.84)} = 17.51, p < 0.0001** Genotype: F_(1,19) = 4.508, p = 0.0471*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.1144 0.04 g: p = 0.1314 0.07 g: p = 0.2376 0.16 g: p > 0.9999 0.4 g: p > 0.9999 0.6 g: p > 0.9999 1 g: p > 0.9999	K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 11
6L	Two-way ANOVA: Time × Genotype: F_(5,95) = 2.362, p = 0.0456* Time: F_{(3.763,71.50)} = 32.01, p < 0.0001**** Genotype: F_(1,19) = 5.763, p = 0.0268*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p > 0.9999 3 d: p = 0.3300 7 d: p = 0.0981 14 d: p > 0.9999 21 d: p = 0.3422 28 d: p = 0.0981	K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 11
7A	Two-way ANOVA: Time × Genotype: F_(5,95) = 3.920, p = 0.0028 Time: F_{(2.941,55.88)} = 26.15, p < 0.0001 Genotype: F_(1,19) = 12.17, p = 0.0025	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p > 0.9999 3 d: p = 0.1218 7 d: p = 0.0531 14 d: p = 0.0300* 21 d: p = 0.0182* 28 d: p = 0.0036**	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
7B	Two-way ANOVA: Time × Genotype: F_(5,95) = 2.145, p = 0.0667 Time: F_{(3.235,61.47)} = 24.76, p < 0.0001** Genotype: F_(1,19) = 10.94, p = 0.0037	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.2213 3 d: p = 0.3591 7 d: p = 0.2636 14 d: p = 0.0280* 21 d: p = 0.0376* 28 d: p = 0.0097**	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
7C	Two-way ANOVA: Time × Genotype: F_(5,100) = 1.356, p = 0.2473 Time: F_{(3.301,66.02)} = 12.18, p < 0.0001**** Genotype: F_(1,20) = 5.713, p = 0.0268*	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p > 0.9999 3 d: p = 0.3348 7 d: p = 0.2794 14 d: p = 0.4035 21 d: p = 0.2362 28 d: p = 0.1361	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
7D	Two-way ANOVA: Time × Genotype: F_(5,100) = 1.418, p = 0.2243 Time: F_{(3.165,63.30)} = 14.29, p < 0.0001**** Genotype: F_(1,20) = 6.443, p = 0.0196*	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.9190 3 d: p = 0.1816 7 d: p = 0.4996 14 d: p = 0.3343 21 d: p = 0.2900 28 d: p = 0.0949	K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
7E	Two-way ANOVA: Time × Genotype: F_(5,110) = 0.04195, p = 0.9990 Time: F_{(2.658,58.48)} = 104.9, p < 0.0001**** Genotype: F_(1,22) = 1.553, p = 0.2258		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
7F	Two-way ANOVA: Time × Genotype: F_(5,110) = 2.042, p = 0.0783 Time: F_{(2.281,50.18)} = 128.6, p < 0.0001**** Genotype: F_(1,22) = 1.528, p = 0.2295		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
7G	Two-way ANOVA: Time × Genotype: F_(5,95) = 0.4997, p = 0.7758 Time: F_{(2.781,52.84)} = 12.16, p < 0.0001**** Genotype: F_(1,19) = 0.8300, p = 0.3737		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 11
8A	Two-way ANOVA: Time × Genotype: F_(5,95) = 0.6338, p = 0.6744 Time: F_{(4.015,76.29)} = 33.68, p < 0.0001**** Genotype: F_(1,19) = 4.186, p = 0.0549		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
8B	Two-way ANOVA: Time × Genotype: F_(5,100) = 0.3192, p = 0.9004 Time: F_{(2.978,59.56)} = 16.05, p < 0.0001**** Genotype: F_(1,20) = 0.0004648, p = 0.9830		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
8C	Two-way ANOVA: Time × Genotype: F_(5,110) = 0.7899, p = 0.5591 Time: F_{(2.437,53.62)} = 49.50, p < 0.0001**** Genotype: F_(1,22) = 1.582, p = 0.2217		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 12
8D	Two-way ANOVA: Time × Genotype: F_(5,55) = 0.8915, p = 0.4932 Time: F_{(3.285,36.14)} = 30.45, p < 0.0001**** Genotype: F_(1,11) = 0.01074, p = 0.9193		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
8E	Two-way ANOVA: Time × Genotype: F_(5,95) = 1.194, p = 0.3180 Time: F_{(4.192,79.65)} = 33.44, p < 0.0001**** Genotype: F_(1,19) = 0.4214, p = 0.5240		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
8F	Two-way ANOVA: Time × Genotype: F_(5,100) = 0.1887, p = 0.9663 Time: F_{(2.440,48.79)} = 39.10, p < 0.0001**** Genotype: F_(1,20) = 0.2250, p = 0.6404		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
9A	Two-way ANOVA: Force × Genotype: F_(6,66) = 1.764, p = 0.1203 Force: F_{(2.812,30.93)} = 44.10, p < 0.0001**** Genotype: F_(1,11) = 0.6411, p = 0.4402		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
9B	Two-way ANOVA: Force × Genotype: F_(6,66) = 1.139, p = 0.3500 Force: F_{(2.157,23.73)} = 8.377, p = 0.0014** Genotype: F_(1,11) = 1.735, p = 0.2146		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
9C	Two-way ANOVA: Time × Genotype: F_(5,55) = 0.9771, p = 0.4400 Time: F_{(3.100,34.10)} = 24.59, p < 0.0001**** Genotype: F_(1,11) = 2.050, p = 0.1800		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
9D	Two-way ANOVA: Force × Genotype: F_(6,114) = 0.06977, p = 0.9986 Force: F_{(2.211,42.01)} = 228.6, p < 0.0001**** Genotype: F_(1,19) = 0.03175, p = 0.8605		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
9E	Two-way ANOVA: Force × Genotype: F_(6,114) = 1.302, p = 0.2620 Force: F_{(2.600,49.40)} = 7.845, p = 0.0004*** Genotype: F_(1,19) = 2.232, p = 0.1516		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
9F	Two-way ANOVA: Time × Genotype: F_(5,95) = 1.758, p = 0.1290 Time: F_{(3.559,67.63)} = 86.98, p < 0.0001**** Genotype: F_(1,19) = 0.1283, p = 0.7241		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
9G	Two-way ANOVA: Force × Genotype: F_(7,140) = 0.5813, p = 0.7703 Force: F_{(2.892,57.84)} = 109.0, p < 0.0001**** Genotype: F_(1,20) = 2.169, p = 0.1564		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
9H	Two-way ANOVA: Force × Genotype: F_(7,140) = 0.9212, p = 0.4921 Force: F_{(1.368,27.37)} = 24.42, p < 0.0001**** Genotype: F_(1,20) = 0.03053, p = 0.8630		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
9I	Two-way ANOVA: Time × Genotype: F_(5,100) = 0.7355, p = 0.5985 Time: F_{(3.709,74.18)} = 57.08, p < 0.0001**** Genotype: F_(1,20) = 1.492, p = 0.2361		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
9J	Two-way ANOVA: Force × Genotype: F_(6,126) = 0.8046, p = 0.5682 Force: F_{(3.100,65.11)} = 125.1, p < 0.0001**** Genotype: F_(1,21) = 0.5743, p = 0.4570		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 11
9K	Two-way ANOVA: Force × Genotype: F_(6,126) = 0.3352, p = 0.9172 Force: F_{(2.521,52.93)} = 16.98, p < 0.0001**** Genotype: F_(1,21) = 0.1460, p = 0.7063		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 11
9L	Two-way ANOVA: Time × Genotype: F_(5,105) = 0.5384, p = 0.7468 Time: F_{(4.229,88.80)} = 42.77, p < 0.0001**** Genotype: F_(1,21) = 0.0004945, p = 0.9825		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 11
10A	Two-way ANOVA: Time × Genotype: F_(5,55) = 0.9608, p = 0.4499 Time: F_{(2.747,30.22)} = 11.41, p < 0.0001**** Genotype: F_(1,11) = 0.6799, p = 0.4271		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
10B	Two-way ANOVA: Time × Genotype: F_(5,55) = 0.9526, p = 0.4548 Time: F_{(2.188,24.07)} = 18.48, p < 0.0001**** Genotype: F_(1,11) = 0.1655, p = 0.6920		K14^C^re⁺;Atoh1^fl^/^fl: 6 K14^C^re^-;Atoh1^fl^/^fl: 7
10C	Two-way ANOVA: Time × Genotype: F_(5,95) = 1.329, p = 0.2585 Time: F_{(2.806,53.32)} = 22.66, p < 0.0001**** Genotype: F_(1,19) = 0.2653, p = 0.6125		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
10D	Two-way ANOVA: Time × Genotype: F_(5,95) = 2.098, p = 0.0723 Time: F_{(3.273,62.18)} = 47.21, p < 0.0001**** Genotype: F_(1,19) = 0.7708, p = 0.3909		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 10
10E	Two-way ANOVA: Time × Genotype: F_(5,100) = 0.4797, p = 0.7907 Time: F_{(1.637,32.74)} = 121.5, p < 0.0001**** Genotype: F_(1,20) = 2.558, p = 0.1254		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
10F	Two-way ANOVA: Time × Genotype: F_(5,100) = 0.4253, p = 0.8301 Time: F_{(3.097,61.93)} = 66.61, p < 0.0001**** Genotype: F_(1,20) = 0.6338, p = 0.4353		K14^C^re⁺;Atoh1^fl^/^fl: 11 K14^C^re^-;Atoh1^fl^/^fl: 11
10G	Two-way ANOVA: Time × Genotype: F_(5,105) = 0.5099, p = 0.7682 Time: F_{(2.937,61.68)} = 8.638, p < 0.0001**** Genotype: F_(1,21) = 0.04139, p = 0.8407		K14^C^re⁺;Atoh1^fl^/^fl: 12 K14^C^re^-;Atoh1^fl^/^fl: 11
11B	Two-way ANOVA: Force × Genotype: F_(7,154) = 0.2821, p = 0.9602 Force: F_{(2.628,57.81)} = 129.8, p < 0.0001**** Genotype: F_(1,22) = 0.07016, p = 0.7936		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11C	Two-way ANOVA: Force × Genotype: F_(7,154) = 1.746, p = 0.1021 Force: F_{(2.464,54.20)} = 165.7, p < 0.0001**** Genotype: F_(1,22) = 2.371, p = 0.1379		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11D	Two-way ANOVA: Time × Genotype: F_(3,66) = 0.8158, p = 0.4897 Time: F_{(2.405,52.90)} = 2.987, p = 0.0498* Genotype: F_(1,22) = 0.8516, p = 0.3661		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11E	Two-way ANOVA: Time × Genotype: F_(3,66) = 1.790, p = 0.1576 Time: F_{(2.210,48.61)} = 31.62, p < 0.0001**** Genotype: F_(1,22) = 1.358, p = 0.2564		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11F	Two-way ANOVA: Time × Genotype: F_(3,66) = 3.104, p = 0.0324* Time: F_{(2.273,50.00)} = 131.0, p < 0.0001**** Genotype: F_(1,22) = 0.4608, p = 0.5044		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11G	Two-way ANOVA: Time × Genotype: F_(3,66) = 0.5619, p = 0.6421 Time: F_{(2.708,59.58)} = 29.72, p < 0.0001**** Genotype: F_(1,22) = 0.3310, p = 0.5709		K14^C^re⁺;Atoh1^fl^/^fl: 13 K14^C^re^-;Atoh1^fl^/^fl: 11
11H	Paired two-tailed t test: t₍₄₎ = 0.04563, p = 0.9658		Contra/Ipsi: 5
Extended Data Fig. 7-1A	Two-way ANOVA: Force × Genotype: F_(6,372) = 5.365, p < 0.0001** Force: F_{(4.151,257.4)} = 227.7, p < 0.0001 Genotype: F_(1,62) = 18.29, p < 0.0001**	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.2758 0.04 g: p = 0.0027 0.07 g: p = 0.0016 0.16 g: p = 0.0002* 0.4 g: p = 0.0067 0.6 g: p = 0.6260 1 g: p = 0.5453	K14^C^re⁺;Atoh1^fl^/^fl: 32 K14^C^re^-;Atoh1^fl^/^fl: 32
Extended Data Fig. 7-1B	Two-way ANOVA: Force × Genotype: F_(6,372) = 8.692, p < 0.0001** Force: F_{(2.545,157.8)} = 62.96, p < 0.0001 Genotype: F_(1,62) = 17.73, p < 0.0001**	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.0001* 0.04 g: p = 0.0004* 0.07 g: p = 0.0010*** 0.16 g: p = 0.0603 0.4 g: p = 0.0566 0.6 g: p = 0.0815 1 g: p = 0.1616	K14^C^re⁺;Atoh1^fl^/^fl: 32 K14^C^re^-;Atoh1^fl^/^fl: 32
Extended Data Fig. 7-1C	Two-way ANOVA: Time × Genotype: F_(5,310) = 5.751, p < 0.0001** Time: F_{(4.383,271.7)} = 62.56, p < 0.0001 Genotype: F_(1,62) = 28.54, p < 0.0001**	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.2364 3 d: p < 0.0001** 7 d: p = 0.0003* 14 d: p = 0.0010 21 d: p = 0.0012 28 d: p = 0.0001***	K14^C^re⁺;Atoh1^fl^/^fl: 32 K14^C^re^-;Atoh1^fl^/^fl: 32
Extended Data Fig. 7-1D	Two-way ANOVA: Time × Genotype: F_(5,310) = 3.217, p = 0.0076 Time: F_{(3.433,212.9)} = 43.18, p < 0.0001 Genotype: F_(1,62) = 13.09, p = 0.0006*	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.1901 3 d: p = 0.0406* 7 d: p = 0.0021** 14 d: p = 0.0126* 21 d: p = 0.0166* 28 d: p = 0.0077**	K14^C^re⁺;Atoh1^fl^/^fl: 32 K14^C^re^-;Atoh1^fl^/^fl: 32
Extended Data Fig. 7-1E	Two-way ANOVA: Time × Genotype: F_(5,205) = 2.975, p = 0.0129* Time: F_{(3.831,157.1)} = 35.77, p < 0.0001** Genotype: F_(1,41) = 17.27, p = 0.0002*	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.0710 3 d: p = 0.0209* 7 d: p = 0.0406* 14 d: p = 0.0041 21 d: p = 0.0057 28 d: p = 0.0005***	K14^C^re⁺;Atoh1^fl^/^fl: 22 K14^C^re^-;Atoh1^fl^/^fl: 21
Extended Data Fig. 7-1F	Two-way ANOVA: Force × Genotype: F_(6,516) = 3.720, p = 0.0012 Force: F_{(4.042,347.6)} = 303.6, p < 0.0001 Genotype: F_(1,86) = 16.28, p = 0.0001*	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p = 0.0307* 0.04 g: p = 0.0010* 0.07 g: p = 0.0046 0.16 g: p = 0.0026 0.4 g: p = 0.0086 0.6 g: p = 0.3399 1g: p = 0.9398	K14^C^re⁺;Atoh1^fl^/^fl: 44 K14^C^re^-;Atoh1^fl^/^fl: 44
Extended Data Fig. 7-1G	Two-way ANOVA: Force × Genotype: F_(6,516) = 13.10, p < 0.0001** Force: F_{(2.425,208.6)} = 89.30, p < 0.0001 Genotype: F_(1,86) = 20.31, p < 0.0001**	Bonferroni multiple comparisons test: K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0.02 g: p < 0.0001** 0.04 g: p < 0.0001 0.07 g: p = 0.0006* 0.16 g: p = 0.0337* 0.4 g: p = 0.0680 0.6 g: p = 0.0912 1 g: p = 0.1714	K14^C^re⁺;Atoh1^fl^/^fl: 44 K14^C^re^-;Atoh1^fl^/^fl: 44
Extended Data Fig. 7-1H	Two-way ANOVA: Time × Genotype: F_(5,430) = 6.981, p < 0.0001** Time: F_{(4.331,372.5)} = 106.6, p < 0.0001 Genotype: F_(1,86) = 37.86, p < 0.0001**	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.0263* 3 d: p < 0.0001** 7 d: p < 0.0001 14 d: p < 0.0001 21 d: p < 0.0001 28 d: p < 0.0001**	K14^C^re⁺;Atoh1^fl^/^fl: 44 K14^C^re^-;Atoh1^fl^/^fl: 44
Extended Data Fig. 7-1I	Two-way ANOVA: Time × Genotype: F_(5,430) = 2.768, p = 0.0178* Time: F_{(3.278,281.9)} = 90.34, p < 0.0001** Genotype: F_(1,86) = 10.54, p = 0.0017	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.1685 3 d: p = 0.0630 7 d: p = 0.0075** 14 d: p = 0.0178* 21 d: p = 0.0297* 28 d: p = 0.0144*	K14^C^re⁺;Atoh1^fl^/^fl: 44 K14^C^re^-;Atoh1^fl^/^fl: 44
Extended Data Fig. 7-1J	Two-way ANOVA: Time × Genotype: F_(5,325) = 2.442, p = 0.0342* Time: F_{(3.575,232.4)} = 85.25, p < 0.0001** Genotype: F_(1,65) = 7.350, p = 0.0086	Bonferroni multiple comparisons test K14^C^re⁺;Atoh1^fl^/^fl vs K14^C^re^-;Atoh1^fl^/^fl 0 d: p = 0.6308 3 d: p = 0.2520 7 d: p = 0.2226 14 d: p = 0.0597 21 d: p = 0.0499* 28 d: p = 0.0152*	K14^C^re⁺;Atoh1^fl^/^fl: 34 K14^C^re^-;Atoh1^fl^/^fl: 33
Extended Data Fig. 7-2A	Two-way ANOVA: Force × Genotype: F_(6,108) = 0.8153, p = 0.5603 Force: F_{(3.220,57.96)} = 271.5, p < 0.0001**** Genotype: F_(1,18) = 0.8246, p = 0.3758		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 10
Extended Data Fig. 7-2B	Two-way ANOVA: Force × Genotype: F_(6,108) = 0.8319, p = 0.5478 Force: F_{(3.457,62.22)} = 3.857, p = 0.0101* Genotype: F_(1,18) = 0.2687, p= 0.6105		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 10
Extended Data Fig. 7-2C	Two-way ANOVA: Time × Genotype: F_(5,90) = 0.8556, p = 0.5143 Time: F_{(2.765,49.76)} = 97.52, p < 0.0001**** Genotype: F_(1,18) = 0.000, p > 0.9999		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 10
Extended Data Fig. 7-2D	Two-way ANOVA: Time × Genotype: F_(5,90) = 0.7883, p = 0.5608 Time: F_{(3.548,63.86)} = 43.28, p < 0.0001**** Genotype: F_(1,18) = 0.1907, p = 0.6675		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 10
Extended Data Fig. 7-2E	Two-way ANOVA: Time × Genotype: F_(5,90) = 0.5307, p = 0.7525 Time: F_{(3.339,60.10)} = 54.82, p < 0.0001**** Genotype: F_(1,18) = 0.4624, p = 0.5052		K14^C^re⁺;Atoh1^fl^/^fl: 10 K14^C^re^-;Atoh1^fl^/^fl: 10
Extended Data Fig. 10-1A	Two-way ANOVA: Force × Genotype: F_(6,330) = 0.1093, p = 0.9953 Force: F_{(3.337,183.5)} = 296.6, p < 0.0001**** Genotype: F_(1,55) = 0.01970, p = 0.8889		K14^C^re⁺;Atoh1^fl^/^fl: 29 K14^C^re^-;Atoh1^fl^/^fl: 28
Extended Data Fig. 10-1B	Two-way ANOVA: Force × Genotype: F_(6,330) = 0.3491, p = 0.9102 Force: F_{(2.745,151.0)} = 30.70, p < 0.0001**** Genotype: F_(1,55) = 0.05107, p = 0.8220		K14^C^re⁺;Atoh1^fl^/^fl: 29 K14^C^re^-;Atoh1^fl^/^fl: 28
Extended Data Fig. 10-1C	Two-way ANOVA: Time × Genotype: F_(5,275) = 0.5749, p = 0.7192 Time: F_{(4.166,229.1)} = 114.4, p < 0.0001**** Genotype: F_(1,55) = 0.09238, p = 0.7623		K14^C^re⁺;Atoh1^fl^/^fl: 29 K14^C^re^-;Atoh1^fl^/^fl: 28
Extended Data Fig. 10-1D	Two-way ANOVA: Time × Genotype: F_(5,275) = 0.3184, p = 0.9017 Time: F_{(3.466,190.7)} = 31.80, p < 0.0001**** Genotype: F_(1,55) = 0.007107, p = 0.9331		K14^C^re⁺;Atoh1^fl^/^fl: 29 K14^C^re^-;Atoh1^fl^/^fl: 28
Extended Data Fig. 10-1E	Two-way ANOVA: Time × Genotype: F_(5,160) = 0.6043, p = 0.6967 Time: F_{(3.064,98.04)} = 53.80, p < 0.0001**** Genotype: F_(1,32) = 0.4431, p = 0.5104		K14^C^re⁺;Atoh1^fl^/^fl: 17 K14^C^re^-;Atoh1^fl^/^fl: 17
Extended Data Fig. 10-1F	Two-way ANOVA: Force × Genotype: F_(6,462) = 0.1290, p = 0.9927 Force: F_{(3.351,258.0)} = 366.2, p < 0.0001**** Genotype: F_(1,77) = 0.2495, p = 0.6188		K14^C^re⁺;Atoh1^fl^/^fl: 40 K14^C^re^-;Atoh1^fl^/^fl: 39
Extended Data Fig. 10-1G	Two-way ANOVA: Force × Genotype: F_(6,462) = 0.6755, p = 0.6696 Force: F_{(2.628,202.4)} = 46.62, p < 0.0001**** Genotype: F_(1,77) = 0.05664, p = 0.8125		K14^C^re⁺;Atoh1^fl^/^fl: 40 K14^C^re^-;Atoh1^fl^/^fl: 39
Extended Data Fig. 10-1H	Two-way ANOVA: Time × Genotype: F_(5,385) = 0.7521, p = 0.5849 Time: F_{(4.238,326.3)} = 164.9, p < 0.0001**** Genotype: F_(1,77) = 0.8046, p = 0.3725		K14^C^re⁺;Atoh1^fl^/^fl: 40 K14^C^re^-;Atoh1^fl^/^fl: 39
Extended Data Fig. 10-1I	Two-way ANOVA: Time × Genotype: F_(5,385) = 0.3927, p = 0.8538 Time: F_{(3.508,270.1)} = 67.12, p < 0.0001**** Genotype: F_(1,77) = 0.1314, p = 0.7180		K14^C^re⁺;Atoh1^fl^/^fl: 40 K14^C^re^-;Atoh1^fl^/^fl: 39
Extended Data Fig. 10-1J	Two-way ANOVA: Time × Genotype: F_(5,270) = 0.3761, p = 0.8649 Time: F_{(3.202,172.9)} = 100.2, p < 0.0001**** Genotype: F_(1,54) = 0.09264, p = 0.7620		K14^C^re⁺;Atoh1^fl^/^fl: 28 K14^C^re^-;Atoh1^fl^/^fl: 28

Asterisks indicated signifcant differences at levels of *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.