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. 2021 Jun 30;41(26):5620–5637. doi: 10.1523/JNEUROSCI.1601-20.2021

Figure 12.

Figure 12.

Anti-inflammatory administration interacts with Ym2 expression and affects cell differentiation in OE colonies. A, Schematic of methimazole and Lenti-shYm2 treatment in OE colonies. B, Schematic of methimazole and Dex administration in OE colonies. C, Quantitative PCR analysis showed decreases in the Ym2-, TNF-α-, and IL-1β-mRNA levels by Ym2 downregulation (n = 3 experiments). D, Quantitative PCR analysis on Ym2-, TNF-α-, and IL-1β-mRNA levels with Dex treatment in OE colonies (n = 3–5 experiments). E, Images of OMP-TdT+ (TdTomato+) colonies treated with saline, 0.1 μm Dex, or 1 μm Dex. F, Statistical analysis of the percentage of OMP-TdT+ colonies in the presence of saline, 0.1 μm Dex, or 1 μm Dex (n = 6, 6, and 7 sections from three independent cultures in each group). Statistical significance was determined by two-way ANOVA with Sidak's multiple-comparisons tests (C), and by one-way ANOVA with Dunnett's multiple-comparisons test (D, F). ns, Not significant. C: F(1,12) = 342.5, p < 0.0001; D: Ym2: F(3,12) = 12.34, p = 0.0004; TNF-α: F(3,10) = 18.15, p = 0.0002; IL-1β: F(3,8) = 5.203, p = 0.0335; F: F(2,16) = 10.83, p = 0.0011. Scale bars, 0.2 mm.