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. 2021 Jun 30;41(26):5620–5637. doi: 10.1523/JNEUROSCI.1601-20.2021

Figure 4.

Figure 4.

Genetic downregulation of Ym2 attenuates regeneration in the lesioned OE. A–L, Immunostaining showed that Ym2 downregulation through tamoxifen (Tam) induction in Sox2-CreERT2/Ym2fl/fl mice decreased the percentage of OMP+ cells (A–D), ICAM1+ cells (E–H), and Sox2+ supporting cells in the OE (I–L) on day 7 and day 31 postinjury compared with oil-injected controls (Ctrl). M, Statistical analysis on the number of OMP+ cells per 100 µm OE on day 7 and day 31 postinjury in control and Ym2 downregulation groups (n = 26 sections from three mice in each group). N, Statistical analysis of the ICAM1 expression intensity on day 7 and day 31 postinjury (n = 12 sections in each group). O, Statistical analysis on the number of apical Sox2+ cells/100 μm OE (n = 15 and 22 sections on day 7 and day 31 postinjury). The dashed line in E–H separated OE from lamina propria. Statistical significance was determined by two-way ANOVA. M: F(1,100) = 24.45, p < 0.0001; N: F(1,44) = 48.70, p < 0.0001; O: F(1,70) =15.16, p = 0.0002. ns, Not significant. Asterisks were determined by Sidak's multiple-comparisons test. Scale bars, 20 μm.