Figure 7.

Presumptive supporting cells in OE colonies express Ym2, and lentiviral infection in OE colonies is not cell type specific. A–F, Confocal images of Ym2⁺ and CK18⁺ (A), Sus4⁺ (B), Tuj1⁺ (C), OMP⁺ (D), ICAM1⁺ (E), and Ki67⁺ (F) cells in OE colonies. G–L, Lentiviral-infected (GFP⁺) OE colonies were immunostained with Sus4 (G), Ym2 (H), ICAM1 (I), Ki67 (J), Sox2 (K), and F4/80 (L). Lenti-Ctrl was used in H; Lenti-shYm2 was used in G, and I–L. Arrowheads mark Ym2⁺/CK18⁺, and Ym2⁺/Sus4⁺ cells in A and B, and positively stained GFP⁺ cells in G–L. Scale bars: C–L, 20 μm; A, B, 10 μm.