Figure 8.

Ym2 regulates cell proliferation of OE colonies. A, B, Quantitative PCR analysis showed the efficiency of Lenti-shYm2 of downregulating Ym2-mRNA expression in Ym2-overexpressed HEK293 cells or OE colonies (n = 3 independent experiments). shYm2-1, shYm2-2, and shYm2-3 were lentiviruses targeting three different parts of the Ym2 sequence. C–F, Confocal images of Ki67⁺ or Sox2⁺ cells in Lenti-shCtrl-, Lenti-shYm2-, Lenti-Ctrl-, or Lenti-Ym2-infected OE colonies. G, H, Statistical analysis of the percentage of Ki67⁺ and Sox2⁺ cells in OE colonies with Lenti-shCtrl, Lenti-shYm2, Lenti-Ctrl, or Lenti-Ym2 infection (n = 12, 12, 6, and 6 sections from three independent cultures, respectively). Statistical significance in A and B was determined by unpaired t test. A: *p = 0.0156, t₍₄₎ = 7.923; B: **p = 0.0013, t₍₄₎ = 27.93; *p = 0.0247, t₍₄₎ = 6.239; and **p = 0.0017, t₍₄₎ = 8.443. Statistical significance was determined by two-way ANOVA in G and H, and asterisks were determined by Sidak's multiple-comparisons test. shCrtl and shYm2: F_(1,44) = 34.59, p < 0.001; Lenti-Ctrl and Lenti-Ym2: F_(1,20) = 9.951, p = 0.005. Scale bars, 20 μm.