Figure 9.

Ym2 regulates cell differentiation in OE colonies. A–D, Confocal images of OMP⁺ cells in colonies infected with Lenti-shCtrl, Lenti-shYm2, Lenti-Ctrl, or Lenti-Ym2. E–H, Immunostaining against Sus4 in Lenti-shCtrl-, Lenti-shYm2-, Lenti-Ctrl-, or Lenti-Ym2-infected colonies. I, Statistical analysis of Sus4⁺ and OMP⁺ cell ratios in colonies with Lenti-shCtrl, Lenti-shYm2, Lenti-Ctrl, or Lenti-Ym2 infection (Sus4⁺: n = 7, 6, 7, and 6 sections from three independent cultures in each group; OMP: n = 6 sections in each group). J–M, Confocal images of Sox2⁺ and Ki67⁺ cells in OE colonies treated with 0, 0.5, 1, and 2 μg/ml Ym2. N–Q, Confocal images of OMP⁺ and Krt14⁺ cells in OE colonies treated with 0, 0.5, 1, and 2 μg/ml Ym2. R, Statistical analysis of Ki67⁺, Sox2⁺, Ki67⁺Sox2⁺, Krt14⁺, and OMP⁺ cells in untreated and recombinant Ym2-treated colonies (Ki67⁺, Sox2⁺, Ki67⁺Sox2⁺: n = 6 sections from three independent cultures in each group; Krt14⁺, OMP⁺: n = 5 sections in Ctrl and 0.5 μg/ml Ym2-treated groups; n = 6 sections in 1 and 2 μg/ml Ym2-treated groups). Statistical significance in I was determined by two-way ANOVA: F_(3,42) = 26.05, p < 0.0001. Asterisks in I were determined by Sidak's multiple-comparisons test. Statistical significance in R was determined by one-way ANOVA, and asterisks were measured by Dunnett's multiple comparisons test. Ki67⁺: F_(3,20) = 5.691, p = 0.0055; Sox2⁺: F_(3,20) = 2.389, p = 0.0991; Ki67⁺Sox2⁺: F_(3,20) = 6.707, p = 0.0026; Krt14⁺: F_(3,18) = 1.696, p = 0.2036; OMP⁺: F_(3,18) = 7.838, p = 0.0015. Scale bars, 10 μm.