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. 2021 Jun 30;7(27):eabg2564. doi: 10.1126/sciadv.abg2564

Fig. 2. Antiandrogen treatments increase cancer cell volume and reduces sphericity in the bone microenvironment.

Fig. 2

(A) Confocal microscopy images of the 3D metastatic microtissues after 24 hours hOBMT coculture with LNCaP and C4-2B under treatments, showing cancer cell morphology (mKO2) on hOBMT (MaxProj. shown, 70-μm z-stacks). (B) Cell segmentation processing through Imaris software. (C and D) 3D morphometric properties of cancer cells after 3 hours and after 24 hours coculture under treatments, shown as box plots, (C) cell volume, and (D) sphericity (>2 microtissues per condition analyzed with >4 random fields of view, average n = 365 cells). DHT (dihydrotestosterone), 10 nM; Enz (enzalutamide), 10 μM; Bic (bicalutamide), 10 μM. *P < 0.05, **P < 0.01, and ****P < 0.0001.