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. 2021 Jun 30;7(27):eabg1037. doi: 10.1126/sciadv.abg1037

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 3 — (A to D) Analytical SEC experiments were performed to demonstrate the interactions between different CENP-C variants and CENP-A^NCPs. Red lines between 1.4 and 1.6 ml of elution volume indicate fractions collected for SDS-PAGE analysis shown in (E). The SEC chromatograms and SDS-PAGEs of all individual components are shown in fig. S5. (E) SDS-PAGE result showing the pooled fractions of the peak 1 complexes shown in the SEC chromatograms in (A) to (D). (F) Sedimentation coefficient distributions obtained from the sedimentation velocity AUC experiments using CENP-A^NCP (left), CENP-A^NCP + ^MBPCENP-C^F-WT (middle), and CENP-A^NCP + ^MBPCENP-C^F-Δmotif2 (right). The table summarizes the results of the three experiments.