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. 2021 Jun 30;7(27):eabg1037. doi: 10.1126/sciadv.abg1037

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 5 — (A) Schematic of the performed amylose-resin pull-down assays. MBP and ^MBPCENP-C^F were immobilized on amylose resin as bait. CENP-TWSX was added as prey, either alone or in combination with CENP-HIKM and/or CENP-LN. (B) Result of the amylose-resin pull-down experiment. The used preys are indicated above each lane. The top gel shows the input fractions, and the bottom gel shows the bound fractions. (C) Graphical summary of the results obtained in (B). (D) SDS-PAGE result showing an amylose-resin pull-down experiment, in which three different Halo-tagged CENP-T variants were used, namely, CENP-T^1–529, CENP-T^1–549, and CENP-T^FL. ^MBPCENP-C was used as bait, and MBP was used as negative control. (E) Representative images showing the fluorescence of ^mCherryCENP-W and three Halo-tagged CENP-T variants labeled with a green fluorescent ligand. Centromeres were visualized by CREST sera, and DNA was stained by DAPI. Scale bars, 10 μm.