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. 2021 Jun 30;7(27):eabd6232. doi: 10.1126/sciadv.abd6232

Fig. 2. S. Typhimurium intestinal burden is higher in Plasmodium coinfected mice despite equivalent bacterial growth.

Fig. 2

Mice infected with P. yoelii (red squares) or mock-infected (white circles) were challenged at 6 dpp with and oral dose of 109 CFU S. Typhimurium invA spiB CmR + pAM34 for assessment of the in vivo replication rate of the pathogen. (A) S. Typhimurium load in the cecal and colon contents 24 hours after inoculation. (B) Generation of the S. Typhimurium population based on pAM34 plasmid retention quantified 24 hours after inoculation. (C) S. Typhimurium load in the cecal and colon contents 4 hours after inoculation. (D) Generation of the S. Typhimurium population based on pAM34 plasmid retention quantified 4 hours after inoculation. Data in (A) and (B) are pooled from two separate experiments for a total of n = 8 mice in the mock group and n = 10 total mice in the P. yoelii–infected group. Data in (C) and (D) represent a single experiment with n = 6 mice per group. Each symbol represents data from one animal. Bars represent the geometric mean. **P ≤ 0.01 and ****P ≤ 0.0001.