Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Caenorhabditis elegans) | JLF631 | This paper; Sallee et al., 2018; Asan et al., 2016; Wang et al., 2015 | ltSi569[TBG-1::mCherry]; zif-1(gk117); wowIs3[ifb-2p::zif-1, myo-2p::mCherry] zuIs70[end-1p::GFP::CAAX] | |
| Strain, strain background (C. elegans) | JLF37 | This paper; Sallee et al., 2018 | zuIs278[pie-1p::mCherry::TBA-1]; gip-1(wow5[ZF::GFP::GIP-1]) zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF83 | This paper; Sallee et al., 2018 | ltSi569; ptrn-1(wow4[PTRN-1::GFP]) | |
| Strain, strain background (C. elegans) | JLF153 | This paper; Sallee et al., 2018 | ltSi569; zif-1(gk117); zyg-9(wow13[ZYG-9::ZF::GFP]) | |
| Strain, strain background (C. elegans) | JJ2376 | CGC, Asako Sugimoto | ddIs6[pie-1p::GFP::TBG-1]; tjIs222[pie-1p::mCherry::AIR-1] | |
| Strain, strain background (C. elegans) | JLF152 | This paper; Sallee et al., 2018 | ltSi569; zif-1(gk117); noca-1(wow11[NOCA-1::ZF::GFP]) | |
| Strain, strain background (C. elegans) | JLF729 | This paper; Sanchez et al., 2020 | ltSi569 vab-10(wow80[VAB-10B::ZF::GFP]); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF878 | This paper; Heppert et al., 2018 | ltSi569; zif-1(gk117); wowIs3; dlg-1(cp301[DLG-1::mNeonGreen]) | |
| Strain, strain background (C. elegans) | JLF719 | This paper; Marston et al., 2016 | hmr-1(cp21[HMR-1::GFP]) ltSi569; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF442 | This paper; Neukomm et al., 2011 | zuIs278; opIs310[ced-1p::YFP::ACT-5] | |
| Strain, strain background (C. elegans) | JLF148 | This paper; CGC | ltSi569 par-6(it319[PAR-6::GFP]); unc-119(ed3 or +) | |
| Strain, strain background (C. elegans) | JLF149 | This paper; CGC | ltSi569; pkc-3(it309[GFP::PKC-3]); unc-119(ed3) or unc-119(+) | |
| Strain, strain background (C. elegans) | JLF147 | This paper; CGC | ltSi569; par-3(it298[PAR-3::GFP]); unc-119(ed3) or unc-119(+) | |
| Strain, strain background (C. elegans) | JLF440 | This paper | ltSi569; zif-1(gk117); wowIs3; ptrn-1(wow4) | |
| Strain, strain background (C. elegans) | JLF445 | This paper | ltSi569 par-6(wow31[PAR-6::ZF::GFP])/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3; ptrn-1(wow4) | |
| Strain, strain background (C. elegans) | JLF492 | This paper | ltSi569; pkc-3(wow85[ZF::GFP::PKC-3])/mIn1[mIs14]; zif-1(gk117); wowIs3; ptrn-1(wow4) | |
| Strain, strain background (C. elegans) | JLF632 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3 zuIs70 | |
| Strain, strain background (C. elegans) | JLF876 | This paper | ltSi569; pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3 zuIs70 | |
| Strain, strain background (C. elegans) | JLF877 | This paper; Zilberman et al., 2017 | ltSi569; cdc-42(xn65[ZF::YFP::CDC-42])/mIn1[mIs14]; zif-1(gk117); wowIs3 zuIs70 | |
| Strain, strain background (C. elegans) | JLF1058 | This paper; Lee et al., 2016 | ltSi569; cdc-25.2(ok597)/oxTi980; zif-1(gk117); wowIs3 zuIs70 | |
| Strain, strain background (C. elegans) | JLF1020 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; cdc-25.2(ok597)/oxTi980; zif-1(gk117)/hT2; wowIs3 zuIs70 | |
| Strain, strain background (C. elegans) | JLF724 | This paper; CGC | ltSi569 par-6(wow31)/tmC27[unc-75(tmIs1239[myo-2p::Venus])]; wowIs28[elt-2p::zif-1, end-1p::histone::mCherry, myo-2p::mCherry; zif-1(gk117)]; opIs310 | |
| Strain, strain background (C. elegans) | JLF784 | This paper | par-3(wow121[PAR-3::tagRFP]) zif-1(gk117); wowIs3; wow4 | |
| Strain, strain background (C. elegans) | JLF785 | This paper | par-6(wow119[PAR-6::ZF::GFP11])/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3; wow4 | |
| Strain, strain background (C. elegans) | JLF720 | This paper | hmr-1(cp21) ltSi569 par-6(wow31)/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF721 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3; dlg-1(cp301) | |
| Strain, strain background (C. elegans) | JLF880 | This paper; Beatty et al., 2010 | ltSi569; itIs256[LGL-1::GFP]; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF881 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; itIs256; zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF879 | This paper; Legouis et al., 2000 | LET-413::GFP par-3(wow121[PAR-3::tagRFP]) zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF817 | This paper | par-6(wow119)/hT2[qIs48]; LET-413::GFP par-3(wow121) zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF204 | This paper | ltSi569; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF1056 | This paper | zif-1(gk117); wowEx175[asp-1p::zif-1; myo-2p::mCherry] | |
| Strain, strain background (C. elegans) | JLF212 | This paper | par-6(wow31); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF758 | This paper | par-6(wow119); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF480 | This paper | pkc-3(wow85); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF882 | This paper | cdc-42(xn65); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF354 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF726 | This paper | par-6(wow119)/hT2[qIs48]; zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF491 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF883 | This paper | ltSi569; cdc-42(xn65)/mIn1[mIs14]; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF1072 | This paper | par-6(wow31)/hT2; zif-1(gk117)/hT2[qIs48]; wowEx175 | |
| Strain, strain background (C. elegans) | JLF1073 | This paper | ltSi569 par-6(wow31)/hT2; zif-1(gk117)/hT2[qIs48]; zuIs70; wowEx175 | |
| Strain, strain background (C. elegans) | JLF866 | This paper | pkc-3(wow85); zif-1(gk117); wowIs3; wowEx143[elt-2p::BFP::PKC-3(+); unc-122p::gfp] ‘line 1’ | |
| Strain, strain background (C. elegans) | JLF867 | This paper | pkc-3(wow85); zif-1(gk117); wowIs3; wowEx144[elt-2p::BFP::PKC-3(+); unc-122p::gfp] ‘line 2’ | |
| Strain, strain background (C. elegans) | JLF870 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx150[elt-2p::BFP::PKC-3(ΔPB); unc-122p::gfp] ‘line 1’ | |
| Strain, strain background (C. elegans) | JLF1044 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx148[elt-2p::BFP::PKC-3(ΔPB); unc-122p::gfp] ‘line 2’ | |
| Strain, strain background (C. elegans) | JLF868 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx146[elt-2p::BFP::PKC-3(G336N); unc-122p::gfp] ‘line 1’ | |
| Strain, strain background (C. elegans) | JLF869 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx147[elt-2p::BFP::PKC-3(G336N); unc-122p::gfp] ‘line 2’ | |
| Strain, strain background (C. elegans) | JLF1074 | This paper | pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx184[elt-2p::BFP::PKC-3(K282A); unc-122p::gfp] ‘line 1’ | |
| Strain, strain background (C. elegans) | JLF895 | This paper | ltSi569; par-3(wow120[PAR-3::GFP]) zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF884 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; par-3(wow120) zif-1(gk117)/hT2; wowIs3 | |
| Strain, strain background (C. elegans) | JLF885 | This paper | ltSi569; pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF877 | This paper | ltSi569; cdc-42(xn65)/mIn1[mIs14]; zif-1(gk117); wowIs3 | |
| Strain, strain background (C. elegans) | JLF1075 | This paper | zif-1(gk117); zuIs70; wowEx175 | |
| Strain, strain background (C. elegans) | JLF1088 | This paper | ltSi569 par-6(wow31)/hT2[qIs48]; zif-1(gk117)/hT2; opIs310; wowEx175 | |
| Strain, strain background (C. elegans) | JLF1076 | This paper | par-6(wow159[PAR-6::RFP]); pkc-3(wow85); zif-1(gk117) | |
| Strain, strain background (C. elegans) | JLF1077 | This paper | par-6(wow159); pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx143[PKC-3(+)] | |
| Strain, strain background (C. elegans) | JLF1078 | This paper | par-6(wow159); pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx148[ΔPB] | |
| Strain, strain background (C. elegans) | JLF1079 | This paper | par-6(wow159); pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx146[PKC-3(G336N)] | |
| Strain, strain background (C. elegans) | JLF1080 | This paper | par-6(wow159); pkc-3(wow85)/mIn1[mIs14]; zif-1(gk117); wowIs3; wowEx184[PKC-3(K282A)] | |
| Recombinant DNA reagent | Plasmid: pDD162 | Addgene | Cas9 + sgRNA plasmid template | |
| Recombinant DNA reagent | Plasmid: pJF250 | Sallee et al., 2018 | ZF::GFP SEC plasmid template | |
| Recombinant DNA reagent | Plasmid: pLC01 | Lauren Cote | ZF::GFP(11) SEC plasmid template | |
| Recombinant DNA reagent | Plasmid: pAL29 | Alex Lessenger, James McGhee | asp-1p::zif-1 gDNA | |
| Recombinant DNA reagent | Plasmid: pCFJ90 | Addgene | myo-2p::mCherry | |
| Recombinant DNA reagent | Plasmid: pMS252 | This paper | elt-2p::bfp::pkc-3(+) gDNA | |
| Sequence-based reagent | oligo: oMS-201-F | This paper | AGCTCGGACACAAGCTCAACagcgctTCGTCTCCGACATCATTAGAGGAG | pkc-3gDNA Fwd with BFP overlap |
| Sequence-based reagent | oligo: oMS202-R | This paper | atgttgaagagtaattggacTCAGACTGAATCTTCCCGACTCATTTG | pkc-3gDNA Rev with unc-54 overlap |
| Recombinant DNA reagent | Plasmid: pMS259 | This paper | elt-2p::bfp::pkc-3[G336N] gDNA | |
| Sequence-based reagent | oligo: oMS-235-F | This paper | ATTCGTTCCTaatGGTGATCTGATG | pkc-3(G336N) Fwd |
| Sequence-based reagent | oligo: oMS-236-R | This paper | TCGATGACAAAGAACAGG | pkc-3(G336N) Rev |
| Recombinant DNA reagent | Plasmid: pMS264 | This paper | elt-2p::bfp::pkc-3[K282A] gDNA | |
| Sequence-based reagent | oligo: oMP215 | This paper | CGCGATAgcAATTATCAAAAA | PKC-3(K282A) Fwd |
| Sequence-based reagent | oligo: oMP216 | This paper | TAAATTTGACGAGTTGAAACATG | PKC-3(K282A) Rev |
| Recombinant DNA reagent | Plasmid: pMS260 | This paper | elt-2p::bfp::pkc-3[deltaPB] gDNA | |
| Sequence-based reagent | oligo: oMP213 | This paper | AAACCAGAGCTGCCCGGG | PKC-3(delta PB) Fwd |
| Sequence-based reagent | oligo: oMP214 | This paper | AGCGCTGTTGAGCTTGTGTC | PKC-3(delta PB) Rev |
| Other | CRISPR Allele DNA reagents: see Supplementary file 1 |