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. 2021 Jun 30;10:e66582. doi: 10.7554/eLife.66582

Figure 1. TRPM4 expression was downregulated in response to left ventricular (LV) pressure overload.

(A) Relative mRNA expression of Trpm4 in LV tissue and (B) in LV cardiomyocytes (CMs) after 2 days and 14 days of sham and TAC. (C) Representative western blots of TRPM4 protein expression in LV tissue (left panel) and in LV cardiomyocytes (right panel). (D) Western blots from LV tissue and (E) LV cardiomyocytes after 2 days and 14 days of TAC were quantified for TRPM4 protein expression. Relative TRPM4 mRNA and protein expression in the LV tissue and cardiomyocytes were normalised by GAPDH and calculated as fold change relative to sham in 2 days and 14 days groups, respectively. Results are presented as means ± SEM. *p<0.05, ***p<0.001 vs. sham-operated groups.

Figure 1—source data 1. Source data file (Excel) for Figure 1A,B,D and E.

Figure 1.

Figure 1—figure supplement 1. Enlarged heart and cardiac fibrosis were detected 14 days after TAC.

Figure 1—figure supplement 1.

(A) Representative photos of hearts from WT mice 2 days or 14 days after sham or TAC. (B) Representative photos of cardiac fibrosis, evaluated by Masson’s trichrome staining of LV tissue from WT mice subjected to TAC versus sham-operated controls. Scale bar = 100 µm.