Figure 1. Ab responses following primary DENV1 or DENV3 infection.

Convalescent sera from people exposed to primary DENV1 (A) or DENV3 (B) infections were tested for NAb before (Control depleted) and after removal of total DENV Abs (Complete Ab depleted) or DENV serotype CR Abs (CR Ab depleted). Total Abs were depleted using DENV serotype responsible for infection as the depleting antigen (DENV1 or DENV3), and CR Abs were removed using a mix of antigen from heterologous DENV serotypes (DENV2 and DENV4 antigens for DENV1 immune sera and DENV1, DENV2, and DENV4 antigens for DENV3 immune sera). Levels of DENV1 or 3 neutralizing Abs were not significantly reduced by the removal of CR Abs. Friedman’s 1-way ANOVA was used to establish statistical significance.