Skip to main content
. 2021 Jul 1;131(13):e143414. doi: 10.1172/JCI143414

Figure 4. Inhibition of PTEN and SAV1 in PACs may activate surrounding macrophages and PSCs via CTGF upregulation in vitro.

Figure 4

(A) mRNA levels of Tnfa, Il1b, and Ccl2 in RAW 264.7 cells 2 days after coculture with 266-6 cells transfected with negative control (NC) siRNA or Cebpa siRNA (#1) (CEBPA knockdown [KD] #1). (B) mRNA levels of Tgfb1, Col1a1, and Col1a2 in PSCs isolated from mouse pancreata 2 days after coculture with 266-6 cells transfected with NC siRNA or Cebpa siRNA (#1) (CEBPA KD#1). (C) mRNA levels of Tnfa, Il1b, and Ccl2 in RAW 264.7 cells 2 days after coculture with 266-6 cells transfected with NC siRNA, Ctgf siRNA (#1) (CTGF KD#1), Cebpa siRNA (#1) (CEBPA KD#1), or both Cebpa (#1) and Ctgf (#1) siRNAs (double KD [DKD] #1). (D) mRNA levels of Tgfb1, Col1a1, and Col1a2 in PSCs isolated from mouse pancreata 2 days after coculture with 266-6 cells transfected with NC siRNA, Ctgf siRNA (#1) (CTGF KD#1), Cebpa siRNA (#1) (CEBPA KD#1), or both Cebpa (#1) and Ctgf (#1) siRNAs (DKD#1). All data are presented as the means ± SDs of results for 3 samples per group. Student’s t test was used to evaluate differences between 2 groups (A and B). One-way ANOVA with Tukey’s post hoc test was used to compare differences among 4 groups (C and D). *P < 0.05 and **P < 0.005.