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. 2021 Jul 1;131(13):e141908. doi: 10.1172/JCI141908

Figure 4. Effect of FGFR depletion on SS cell growth.

Figure 4

Immunoblots show FGFR1, FGFR2, FGFR3, ETV4, and ETV5 expression in SYO-1 cells expressing shFGFR1 (A), shFGFR2 (C), and shFGFR3 (E). Control is non-target (TRC2-PLKO.1) vector. shFs represent specific short hairpin FGFR-targeting vectors; -1 and -2 indicate different vectors. Actin served as loading control in all immunoblots. n = 2. The vertical lines in the ETV4, ETV5, and actin immunoblots designate nonconsecutive lanes of the same SDS-PAGE gels. Bar graphs show the effect of FGFR1 (B), FGFR2 (D), and FGFR3 (F) lentiviral depletion on SYO-1 cell growth on day 2 and day 4 after infection. Growth was measured by absorbance at 570 nm. The 10 overlaid dots in each graph represent independent values from 2 sulforhodamine B (SRB) assays conducted in 5 replicates each. Error bars indicate SEM. P values using paired t test compare mean absorbance in each shF group against mean absorbance in the control vector (TRC2) group. *P < 0.00001 in all graphs. ANOVA F ratios and corresponding P values shown on top of each graph demonstrate significant variation of means among the 3 groups on each day.