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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nature. 2021 Feb 3;591(7851):665–670. doi: 10.1038/s41586-020-03150-2

Extended Data Figure 10 |. Overexpression of ActB- or Casp16p-sdRNA results in DNA damage at the pause site of the gene that encoded it.

Extended Data Figure 10 |

a, DNA damage quantitation at ACTB and CASP16P termination pause sites following ActB- or Casp16p-sdRNA overexpression (OE) using γ-H2AX ChIP qPCR analyses. The ChIP signal reflects the average fold change over the untransfected condition (control) (n = 4–6 biological replicates). Data were analyzed by Two-way Anova with post-hoc Tukey HSD and compared to the relevant control cells. b, Model of sdRNA-mediated damage repair at R-loop positive pause sites in cycling and quiescent cells.