Extended Data Figure 2 |. BRCA1 interacts with Dicer and Ago1/2 to prevent termination pause site damage throughout the cell cycle.
a-b, Immunoblots of Cyclin A and BRCA1 from WCE of FUCCI HeLa cells sorted according to the expression of the G1 marker, mKO2-hCdt1 (a) or the S/G2 phase mAG-hGeminin marker (b); n=4. c, Cell cycle- dependent analysis of DNA damage using γ-H2AX ChIP signals in HeLa FUCCI sorted according the S/G2 hGeminin marker indicating that G1 cells develop more damage than S/G2 cells. A representative experiment is shown, and the histograms depict the average fold change of qPCR replicates ± s.d. d, Immunoblots depicting the expression of BRCA1 during the various phases of the cell cycle upon release from serum starvation-induced G0 T98G cells; n=5. e, DNA damage analysis of synchronized T98G cells. f, Immunoblots validating the re-expression of BRCA1 (R) or Dicer (Flag-tagged WT and mutant Dicer, detected with LiCor and showing their quantitative expression) after BRCA1 or Dicer depletion, respectively in HMECs and HeLa cells; n=4. Data in c were analyzed by One-way Anova with post-hoc Tukey HSD and compared to an undamaged locus.