Skip to main content
. 2021 Jun 23;2021:5554569. doi: 10.1155/2021/5554569

Figure 2.

Figure 2

Effect of Ginkgolide B on autophagy in Ang II-induced cardiomyocytes. (a) Fluorescence microscopy of H9c2 cells staining with an LC3-II antibody (green, magnification ×100). (b) Quantification of cells with LC3-II dots. (c) Representative blots of autophagy-related proteins. GB further increases the ratio of LC3-II/LC3-I (d) and Beclin-1 protein expression (e) and decreases the P62 protein (f) in H9c2 cells with Ang II. Cells were pretreated with the autophagy inhibitor, 3-MA (1 μM). ∗∗P < 0.01, ∗∗∗P < 0.001 vs. the control group; #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. the Ang II group. (g) 3-MA significantly attenuates Ginkgolide B-induced inhibition in ANP mRNA expression. ∗∗∗P < 0.001 vs. the GB group; ##P < 0.01 vs. the 3-MA group.