Fig. 4. Validation of novel nuclear G4-selective binding proteins.
a, Affinity enrichment coupled with western blot analysis of selected candidates for different topologies of G4 structures and control oligonucleotides (G-runs are highlighted in bold). A representative blot from two independent experiments with similar results is shown. b–e, Binding curves (the indicated Kd values were generated by ELISA) for the human recombinant full-length SMARCA4 protein to G4 Kit1, the single-stranded mutant (ss mutKit1) and double-stranded Kit1 (ds Kt1) (b), UHRF1 protein to G4 Kit1, ss mutKit1, Kit1 hemi-methylated dsDNA and ds Kit1 (c), DDX1 protein to G4 Myc, ss mutMyc and ds Myc (d), DDX24 protein to G4 Kit1, ss mutKit1 and ds Kit1 (e) and RBM22 protein to G4 NRAS and its mutant (mutNRAS) (f). Mean and error (± s.d.) are from three independent experiments (n = 3). a.u., arbitrary units.