Extended Data Fig. 4. Protein validation by affinity enrichment coupled with western blot analysis and ELISA.
a, Affinity enrichment coupled with western blot analysis of HMGB2 for different topologies of G4 structures and control oligonucleotides. A representative blot from two independent experiments with similar results is shown. Structure verification of G4 Myc (b) and G4 Kit1 (c) and the indicated control oligonucleotides with CD spectroscopy. Curves are plotted by mean values of three independent experiments (n = 3). d, Binding curves with indicated dissociation constants (Kd) generated by ELISA for human recombinant full-length RBM22 protein to DNA G4 Myc, single-stranded mutant and Myc duplex DNA. Mean and error (± S.D.) are represented from three independent experiments (n = 3). G-runs are highlighted in bold.