Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 2;473(7):1009–1021. doi: 10.1007/s00424-021-02571-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — hiPSC-CMs express HCN isoforms and auxiliary proteins. a Box-plot showing qRT-PCR analysis of HCN1-4 genes in iPSC-CMs at d15, 30, and 60, as indicated; Troponin T expression was used as reference gene in each sample to normalize for cardiomyocyte yield in different differentiations. b qRT-PCR analysis of genes known to be f-channels auxiliary subunits (caveolin-3-CAV3; Alpha-1,2-Glucosyltransferase-KCR1; Discs large homolog 1-SAP97; and Potassium channel βsubunit-MIRP1) in beating cultures at the various time points. c WB analysis of HCN4 and HCN1 in three hiPS-CM cultures at the various time points. cTnT expression was used for estimating content in cardiomyocytes. d Confocal microscopy image of hiPSC-CM showing co-expression of HCN4 (green) and caveolin-3 (red); nuclei were counterstained with DAPI (blue)