Fig. 5.

Spontaneous rate of pCMs is modulated by sympathetic and parasympathetic agonists. a Representative time course of the action potential rate in clusters of pCMs at 30 days of differentiation before, during 1 µM isoproterenol perfusion, and after washout, as indicated. b Representative time course of the action potential rate before, during, and after washout of 100 nM acetylcholine (ACh) perfusion. c, d Representative action potential traces recorded before (solid line) and during (dashed line) isoproterenol (c) and Ach (d) stimulation; traces correspond to points 1 and 2 in of the respective time courses. e, f Dot plot of the percentage change in firing rate during 1 µM Iso (e) and 100 nM Ach (f). Mean ± SEM value is reported as filled square and whiskers