Table 1.
yeastDrop-Seq solution.
| Solution (total volume) | Reagent | Reagent volume |
|---|---|---|
| yeastDrop-seq solution (2223.5 μL) | Zymolyase (Zymo Research E1004), 2 Units/μL | 37.5 μL |
| Lysis buffer (no DTT) | 1.5 mL | |
| 1 M DTT | 75 μL | |
| Zymolyase buffer | 600 μL | |
| 10% SDS (final concentration at 0.05%) | 11 μL | |
| Lysis buffer (no DTT)2 (950 μL) | H2O | 500 μL |
| 20% Ficoll PM-100 | 300 μL | |
| Sarkosyl | 10 μL | |
| 0.5 M EDTA | 40 μL | |
| 2 M Tris pH 7.5 | 100 μL | |
| Zymolyase buffer37 (CSH protocols Zymolyase Buffer) (100 mL) | 2 M Sorbitol | 50 mL |
| 1 M K2HPO4 | 4.2 mL | |
| 1 M KH2PO4 | 0.8 mL | |
| EDTA (0.5 M, pH 8) | 1 mL | |
| H2O | 44 mL |