Fig. 3.

Anti-ASGM1 treatment abolished the ability to clear HBV in NFIL3 knockout (KO) mice. a, b NFIL3 KO mice underwent hydrodynamic injection (HDI) with adeno-associated virus (AAV)/HBV1.2 plasmids in the presence or absence of anti-ASGM1 treatment (n = 10 for each group). Anti-ASGM1 treatment was performed twice per week over the detection period. Serum titers of HBsAg (a) and anti-HBs Ab (b) at the indicated times were determined by ELISA. (c) IHC staining for detection of HBcAg expression in liver tissues (arrow) in NFIL3 KO mice with or without anti-ASGM1 treatment on days 3 and 42 after HDI. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 between selected relevant comparisons by Student’s t-test