Table 1.
Mac98A/Nup116 FG phases stained by fluorescent aromatic compounds (complete list).
| Environmentally insensitive fluorophores | TtMac98A | scNup116 |
|---|---|---|
| FG in:out | FG in:out | |
| Abberior STAR 635P* | 16 | N.D. |
| Alexa488* | 6.3 | 36 |
| Alexa568* | 9.2 | 11 |
| Alexa594* | 21 | 25 |
| Alexa647* | 8.5 | 13 |
| Atto488* | 2.0 | 2.0 |
| Atto565* | 51 | 47 |
| Atto647N* | 60 | 110 |
| Cy3* | 110 | 610 |
| Cy5* | 88 | 180 |
| DyLight405* | 16 | N.D. |
| DyLight488* | 3.7 | N.D. |
| Fluorescein | 7.4 | N.D. |
| Fluorescein* | 8.7 | 10.3 |
| OregonGreen488* | 12 | N.D. |
| Rhodamine B | 37 | 110 |
| Sulfo-Cy3* | 66 | 130 |
| Sulfo-Cy5* | 34 | 28 |
| Environmentally sensitive fluorophores | TtMac98A | scNup116 |
|---|---|---|
| FG in:out | FG in:out | |
| DAPI | 1700 | 8100 |
| Ethidium bromide | 22 | N.D. |
| Hoechst 33258 | 3300 | N.D. |
| Hoechst 33342 | 3300 | >10000 |
| Hoechst 34580 | >10000 | >10000 |
| Nuclear yellow | 970 | N.D. |
| Oxazole yellow | 380 | N.D. |
| SYPRO orange | 1000 | 1090 |
| Thioflavin-T | 4.3 | 220 |
N.D. not determined.
The fluorophores are arranged in alphabetical order. * indicates that a cysteine-quenched fluorophore maleimide was tested.