Fig. 1. Specific expression of the transcription factor gene VqMYB154 under artificial inoculation conditions using U. necator and other exogenous treatments.
a VqMYB154 was screened from 106 MYB genes in Danfeng-2 leaves infected by U. necator artificial inoculation under field conditions. b–e Expression of MYB154 was determined by qRT-PCR. b Leaves of Danfeng-2 and (c) Cabernet Sauvignon were inoculated with U. necator and sampled at 0, 12, 24, 48, 72, 96, and 120 h. d Leaves of Danfeng-2 and (e) Cabernet Sauvignon were inoculated with Pst DC3000 and collected at 0, 24, 48, 72 h. Pst DC3000, Pst DC3000 infection; PM-Inoculation, U. necator infection; Mock, control treated with sterile water. f Leaves from Danfeng-2 were treated with 100 μM phytohormones, 1% (w/v) hydrogen peroxide, and 5 mM CaCl2. Leaves were collected at 0, 0.5, 1, 2, 6, and 10 h after spraying. Results are shown as the means (±SD) of three biological assays. Significance was determined with GraphPad Prism using one-way ANOVA with Fisher’s LSD test (*P < 0.05; **P < 0.01)