Fig. 3. Location and structural analysis of VqMYB154 isolated from V. quinquangularis accession Danfeng-2.

a Chromosomal location of VqMYB154. VqMYB154 is located on chromosome 11, from position 2193249 to 2194450. The MYB domain is located at the N-terminus of VqMYB154, from aa 13 to aa 115. b Amino acid sequence alignments between VqMYB154 and VvMYB154. Differences are marked with a red box. c Multiple sequence alignments among VqMYB154 and its homologs in other species. The R2 MYB domain is indicated with a light blue line, and the R3 domain is shown with a dark blue line. The sequences are from the following: VvMYB154 (XP_002280054.2), AtMYB36 (OAO95809.1), NtRAX2 (XP_016474887.1), SlRAX3 (XP_004238554.1), OsRAX2 (XP_015637875.1), ZmMYB4 (XP_008656763.1), and MdMYB36 (XP_008337621.2). d Cluster analysis of VqMYB154 with its homologous genes. VqMYB154 is marked with a black triangle. e Cluster analysis of VqMYB154 with MYB proteins in grape, Arabidopsis, and rice. VqMYB154 belongs to subgroup 14 (S14) and is highly homologous to VvMYB154, VvMYB153, OsMYB12, and OsMYB72. VqMYB154 is highlighted in red and shown with a red arrow. f The location of VqMYB154 is in the nucleus. GFP was ligated to VqMYB154 (VqMYB154-GFP), and mCherry was ligated to the Arabidopsis nuclear localization marker AtHY5 (AtHY5-mCherry). The mixed plasmids were transformed together into Arabidopsis protoplasts. 35S-GFP was adopted as the control. The GFP and mCherry signals were detected with a confocal microscope. Bars: 5 μm. g Transactivation assay of VqMYB154 in yeast. Yeast strains harboring BD-VqMYB154 and the BD control were grown on SD/-Trp, SD/-Trp with AbA, and SD/-Trp with AbA and X-α-Gal medium and cultivated at 28 °C for 3 days. Only the yeast strain of BD-VqMYB154 grew and turned blue on SD/-Trp with AbA and X-α-Gal medium