Standard curve and melt curve specific to ICRSV obtained by a SYBR Green RT-qPCR assay using tenfold serial dilutions of moles of cRNA standard ranging from 9.481 fmol, 948.1 amol, 94.81 amol, 9.481 amol, 0.9481 amol, 0.09481 amol, 0.009481 amol, 0.0009481 amol, and 0.00009481 amol