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Sensitivity of coat protein gene based developed assay: A Amplification plot of RT-qPCR assay using SYBR Green dye. B Reverse transcription polymerase chain reaction (RT-PCR). Lane M, 100 bp DNA Ladder; lane 2 to 9, indicate the cRNA concentration in RT-qPCR assay starting from 5.709 × 109 to subsequent tenfold dilution up to 5.709 × 101, respectively; and Lane NTC, non-template control
