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Validation of RT-qPCR assay using RT-PCR of few representative field samples: A Amplified PCR product of ICRSV coat protein gene; B Amplified PCR product of ICRSV nucleic acid binding gene: Lane M, 100 bp Ladder; Lane 1–8, Field samples; Lane C1–C2, Healthy Plant; Lane −Ve, non-template control; and Lane + Ve, ICRSV positive control
