Table 1.

Primer list used in assay standardization

Target gene	Assay	Primer code	Primer sequence (5′–3′)	Amplicon size (bp)
ICRSV coat protein	RT-qPCR (target)	ICRSV-RT-1F	GCTAACCCTAAACAGACCGAG	211
		ICRSV-RT-1R	GGCAGAAATCCCTAACCACTAG
		ICRSV-RT-2F	TATACGCCATTGACCTCGCC	289
		ICRSV-RT-2R	GAAGCTGGCGAGAGGATACC
		ICRSV-RT3-F	GCGCTTTTTGCGCTTACTAC	164
		ICRSV-RT3-R	TAGGGGCACATACAGGGAAG
Plant cytochrome oxidase	RT-qPCR (internal control)	COX-F	F GTATGCCACGTCGCATTCCAGA	68
		COX-R	GCCAAAACTGCTAAGGGCATTC
Citrus sinensis elongation factor 1alpha		EF-1α- F	CTCAAGCCTGGTATGGTGGT	185
		EF-1α -R	GGATCATCCTTCGAGTTGGA
T7 Promoter containing ICRSV Coat protein	RT-PCR (cRNA)	T7-ICRSV-CP-F	TAATACGACTCACTATAGGGAGA ATGAGCTTTGACTACAC	988
		ICRSV-CP-3R	TCATTAGATGTTGAAAGGGGTC
ICRSV Coat protein	RT-PCR	ICRSV-CP-3F	AGCTTTGACTACACACACC	978
		ICRSV-CP-3R	TCATTAGATGTTGAAAGGGGTC
Nucleic acid binding region		NAB-F	TGGAACCTCATGATCAAAGCG	668
		NAB-R	TCATCTAGGGTCGAGGAGC