Table 2.
Detection and quantification of Indian citrus ringspot virus in biologically indexed plants along with field-collected citrus samples, and in samples of citrus trees known to be infected by other major citrus pathogens using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription polymerase chain reaction (RT-PCR)
| Sr. No. | Sample code | Citrus cultivar | Scientific name | RT-qPCR | PCR with primer pairs | ||
|---|---|---|---|---|---|---|---|
| Ct values | Log 10 values of virus copy number/500 ng RNA | ICRSV-CP-3F/CP-3R | NAB-F/NAB-R | ||||
| Biological indexing | |||||||
| 1 | ICRSV-K-1 | Kinnow | C. reticulata | 16.25 | 5.11 | + | + |
| 2 | ICRSV-K-2 | Kinnow | C. reticulata | 16.55 | 5.03 | + | + |
| 3 | ICRSV-K-3 | Kinnow | C. reticulata | 17.12 | 4.86 | + | + |
| 4 | ICRSV-M-4 | Mosambi | C. sinensis | 20.12 | 4.02 | + | + |
| 5 | ICRSV-K-5 | Kinnow | C. reticulata | 18.56 | 4.46 | + | + |
| 6 | ICRSV-K-6 | Kinnow | C. reticulata | 19.36 | 4.23 | + | + |
| 7 | ICRSV-K-7 | Kinnow | C. reticulata | 20.35 | 3.95 | + | + |
| 8 | ICRSV-M-8 | Mosambi | C. sinensis | 21.23 | 3.70 | + | + |
| 9 | ICRSV-M-9 | Mosambi | C. sinensis | 19.63 | 4.15 | + | + |
| 10 | ICRSV-M-10 | Mosambi | C. sinensis | 25.06 | 2.62 | + | + |
| 11 | ICRSV-K-11 | Kinnow | C. reticulata | 21.65 | 3.58 | + | + |
| Field collected citrus samples | |||||||
| 12 | P-K-LUH-1 | Kinnow | C. reticulata | 22.12 | 3.45 | + | + |
| 13 | P-K-LUH-2 | Kinnow | C. reticulata | UD | UD | − | − |
| 14 | P-K-LUH-3 | Kinnow | C. reticulata | 18.12 | 4.58 | + | + |
| 15 | P-D-LUH-4 | Daisy | C. reticulata | 17.39 | 4.79 | + | + |
| 16 | P-K-LUH-5 | Kinnow | C. reticulata | UD | − | − | − |
| 17 | P-G-LUH-6 | Grapefruit | C. paradisi | 16.64 | 5.00 | + | + |
| 18 | P-G-LUH-7 | Grapefruit | C. paradisi | 17.62 | 4.72 | + | + |
| 19 | P-P-LUH-8 | Pomelo | C. grandis | UD | − | − | − |
| 20 | P-P-LUH-9 | Pomelo | C. grandis | 21.08 | 3.74 | + | + |
| 21 | P-K-LUH-10 | Kinnow | C. reticulata | UD | − | − | − |
| 22 | P-K-HSP-11 | Kinnow | C. reticulata | 22.12 | 3.45 | + | + |
| 23 | P-K-HSP-12 | Kinnow | C. reticulata | UD | − | − | − |
| 24 | P-K-HSP-13 | Kinnow | C. reticulata | UD | − | − | − |
| 25 | P-K-HSP-14 | Kinnow | C. reticulata | 19.2 | 4.28 | + | + |
| 26 | P-K-HSP-15 | Kinnow | C. reticulata | 18.33 | 4.52 | + | + |
| 27 | P-K-ABH-16 | Kinnow | C. reticulata | UD | − | − | − |
| 28 | P-K-ABH-18 | Kinnow | C. reticulata | UD | − | − | − |
| 29 | P-K-ABH-19 | Kinnow | C. reticulata | 19.21 | 4.27 | + | + |
| 30 | P-M-ABH-20 | Malta | C. sinensis | 17.32 | 4.81 | + | + |
| 31 | P-M-ABH-21 | Malta | C. sinensis | UD | − | − | − |
| 32 | P-K-ABH-22 | Kinnow | C. reticulata | 25.21 | 2.57 | + | + |
| 33 | P-K-ABH-23 | Kinnow | C. reticulata | UD | − | − | − |
| 34 | P-K-ABH-24 | Kinnow | C. reticulata | 23.78 | 2.98 | + | + |
| 35 | P-K-ABH-25 | Kinnow | C. reticulata | 20.44 | 3.92 | + | + |
| 36 | P-K-ABH-26 | Kinnow | C. reticulata | 19.65 | 4.15 | + | + |
| 37 | P-K-ABH-27 | Kinnow | C. reticulata | UD | − | − | − |
| 38 | P-K-ABH-28 | Kinnow | C. reticulata | 18.56 | 4.46 | + | + |
| 39 | P-K-ABH-29 | Kinnow | C. reticulata | 20.87 | 3.80 | + | + |
| 40 | P-K-ABH-30 | Kinnow | C. reticulata | 21.63 | 3.59 | + | + |
| 41 | P-K-MUK-31 | Kinnow | C. reticulata | UD | − | − | − |
| 42 | P-K-MUK-32 | Kinnow | C. reticulata | 17.39 | 4.79 | + | + |
| 43 | P-K-MUK-33 | Kinnow | C. reticulata | UD | − | − | − |
| 44 | P-K-MUK-34 | Kinnow | C. reticulata | 25.29 | 2.57 | + | + |
| 45 | P-K-MUK-35 | Kinnow | C. reticulata | 21.87 | 3.52 | + | + |
| 46 | P-K-MUK-36 | Kinnow | C. reticulata | UD | − | − | − |
| 47 | P-K-BTI-37 | Kinnow | C. reticulata | 20.56 | 3.89 | + | + |
| 48 | P-K-BTI-38 | Kinnow | C. reticulata | 25.62 | 2.46 | + | + |
| 49 | P-K-BTI-39 | Kinnow | C. reticulata | 22.66 | 3.30 | + | + |
| 50 | P-K-BTI-40 | Kinnow | C. reticulata | UD | − | − | − |
| 51 | P-K-FAZ-41 | Kinnow | C. reticulata | 24.61 | 2.74 | + | + |
| 52 | P-K-FAZ-42 | Kinnow | C. reticulata | 21.69 | 3.57 | + | + |
| 53 | P-K-FAZ-43 | Kinnow | C. reticulata | UD | − | − | − |
| 54 | H-K-SIR-44 | Kinnow | C. reticulata | 25.63 | 2.46 | + | + |
| 55 | H-K-SIR-45 | Kinnow | C. reticulata | UD | − | − | − |
| 56 | H-K-SIR-46 | Kinnow | C. reticulata | UD | − | − | − |
| 57 | H-K-SIR-47 | Kinnow | C. reticulata | 24.33 | 2.82 | + | + |
| 58 | H-K-SIR-48 | Kinnow | C. reticulata | 30.21 | 1.16 | + | + |
| 59 | H-K-SIR-49 | Kinnow | C. reticulata | 25.31 | 2.55 | + | + |
| 60 | MH-M-1 (Healthy) | Mosambi | C. sinensis | UD | − | − | − |
| 61 | MH-M-2 (Healthy) | Mosambi | C. sinensis | UD | − | − | − |
| Other major citrus pathogens | |||||||
| 62 | AL-1 (CTV) | Acid lime | C. aurantifolia | UD | − | − | − |
| 63 | M-1 (CTV) | Mosambi | C. sinensis | UD | − | − | − |
| 64 | K-1 (CYVCV) | Kinnow | C. reticulata | UD | − | − | − |
| 65 | K-2 (CYVCV) | Kinnow | C. reticulata | UD | − | − | − |
| 66 | M-1 (CYMV) | Mosambi | C. sinensis | UD | − | − | − |
| 67 | M-2 (CYMV) | Mosambi | C. sinensis | UD | − | − | − |
| 68 | AL-(CLas) | Acid lime | C. aurantifolia | UD | − | − | − |
| 69 | NM-1 (CLas) | Mandarin | C. reticulata | UD | − | − | − |
| 70 | AL-1 (citrus phytoplasma) | Acid lime | C. aurantifolia | UD | − | − | − |
| 71 | AL-2 (citrus phytoplasma) | Acid lime | C. aurantifolia | UD | − | − | − |
UD undetermined