Fig. 3.

eNKs exert a strong cytotoxic effect on Dvd-pretreated tumor cells. A Percentage of CD107a-expressing eNKs (CD3⁻CD56⁺) after coculture with Dvd-pretreated K562, U266, RPMI8226, or Raji cells (E:T ratio of 1:1) for 4 h. Flow cytometry revealed a higher level of CD107a in eNKs cocultured with Dvd-treated tumor cells. B The ADCC effect of Dara on K562, U266, RPMI8226, and Raji cells determined by a standard flow cytometry-based cytotoxicity assay. Dara significantly induced ADCC in RPMI8226 and Raji cells at all E:T ratios. C eNK-mediated cytotoxicity in Dvd-pretreated tumor cells (K562, U266, RPMI8226, and Raji) measured by a standard flow cytometry-based cytotoxicity assay. eNKs showed potent antitumor activity against Dvd-pretreated tumor cells at all E:T ratios. *p < 0.05, **p < 0.001, and ***p < 0.0001