Fig. 6.

Dvd treatment enhances eNK homing in vivo in RPMI8226-RFP-FLuc-bearing mice. A In vivo homing of eNKs in RPMI8226-RFP-FLuc-bearing mice (n = 9 mice per group) evaluated by flow cytometry. Mice were sacrificed at the experimental endpoint, and BM samples were analyzed by flow cytometry for human NK cell (CD3⁻CD56⁺) and activation receptor (CD16 and NKG2D) levels. B Quantification (mean ± SD) of in vivo eNK homing in the BM based on flow cytometry data. eNKs from mice treated with Dvd + eNKs showed the strongest in vivo homing ability. C Immunohistochemical staining for human CD56, CD16, and NKG2D in femur and tibia sections (no treatment, day 36; Dara, day 60; eNKs, day 63; Dara + eNKs, day 75; Dvd and Dvd + eNKs, day 91). The BM of mice treated with Dvd + eNKs had the highest staining intensity for NK cell markers (magnification: ×10 for hematoxylin and eosin; ×600 for CD56, CD16, and NKG2D immunostaining. *p < 0.01, **p < 0.001, ***p < 0.0001