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. 2021 Jul 1;8:156. doi: 10.1038/s41438-021-00590-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Yeast one-hybrid (Y1H) analysis of interactions of MdMYB90-like (left panels) and MdMYB1 (middle panels) with the promoters of anthocyanin biosynthesis-related genes. The pB42AD vector was used as the negative control (right panels). B Dual-luciferase detection experiments showed that MdMYB90-like promoted the expression of the MdCHS, MdBHLH3, MdUFGT, and MdMYB1 genes. C LUC/REN activities of constructs: 35Spro:MdMYB90-like/MdMYB1pro:LUC and 35Spro:62-SK/MdMYB1pro:LUC; 35Spro:MdMYB90-like/MdbHLH3pro:LUC and 35Spro:62-SK-MdbHLH3pro:LUC; 35Spro:MdMYB90-like/MdUFGTpro:LUC and 35Spro:62-SK-MdUFGTpro:LUC; 35Spro:MdMYB90-like/MdCHSpro:LUC and 35Spro:62-SK-MdCHSpro:LUC