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. 2021 Jul 1;8:159. doi: 10.1038/s41438-021-00593-0

Fig. 1. MdZAT10 promotes leaf senescence.

Fig. 1

a MdZAT10 transcript level in late-senescent (LS), early-senescent (ES), and nonsenescent (NS) leaves. b Detached leaves from 3-week-old wild-type (Col) plants and three transgenic lines (MdZAT10-L1, L2, and L3) were incubated in 3 mM MES buffer in the dark for 5 days. c The chlorophyll content and d Fv/Fm before and after dark treatment were determined. e Leaf senescence phenotype and f the total chlorophyll content of apple leaves transiently expressing empty vector (EV), overexpressing MdZAT10 or MdZAT10 antisense vector in the dark for 15 days. g, h The expression levels of MdNYC1 and MdNYE1 in apple leaves after 15 days of dark treatment. Asterisks indicate significant differences determined by t-test (*P < 0.05, **P < 0.01)