Fig. 2. MdZAT10 physically interacts with MdABI5.

a A yeast two-hybrid (Y2H) assay showed that MdZAT10 interacts with MdABI5. Full-length MdZAT10 and truncated MdZAT10 sequences were cloned into the pGAD424 vector. Full-length MdABI5 was cloned into the pGBT9 vector. The empty pGAD vector was used as a negative control. b A Y2H assay showed that MdABIs (MdABI1, MdABI2, and MdABI4) and MdZAT10 interact. c A Y2H assay showed that MdABI5 interacts with MdZAT proteins (MdAZF1, MdZAT5, MdZAT6, MdZAT10, MdZAT11, MdZAT14, MdZAT16, and MdZAT18). d In vitro MdZAT10 and MdABI5 pull-down assay. The MdABI5-His protein was incubated with MdZAT10-GST and GST. Proteins pulled down with GST beads were detected using anti-GST and anti-His antibodies. e BiFC assay. The MdZAT10-cYFP and MdABI5-nYFP constructs were transiently expressed in Nicotiana benthamiana leaves, and the fluorescence signal was observed by fluorescence microscopy. Nuclei are indicated by DAPI staining. Scale bars, 10 μm