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. 2021 Jul 1;8:159. doi: 10.1038/s41438-021-00593-0

Fig. 3. MdZAT10 accelerates MdABI5-regulated leaf senescence.

Fig. 3

a Leaf senescence phenotype and b total chlorophyll content of detached leaves from Col, MdABI5-L2, and MdZAT10-L1/MdABI5-L2 transgenic Arabidopsis floated on 3 mM MES buffer in the dark for 5 days. c Leaf senescence phenotype and d total chlorophyll content of apple leaves transiently expressing empty vector (EV), overexpressing MdABI5 alone or overexpressing MdZAT10 and MdABI5 that were floated on 3 mM MES buffer in the dark for 14 days. e, f The expression levels of MdNYC1 and MdNYE1 in wild-type (WT) calli and MdABI5-OX, MdZAT10-OX, MdZAT10-OX/MdABI5-OX transgenic calli were measured by qRT-PCR assay. g Schematic diagram of reporter and effector constructs. h, i A luciferase assay showed that transient cotransformation of MdZAT10 and MdABI5 into tobacco leaves activated the expression of MdNYC1 and MdNYE1. The empty vector (SK + LUC) served as a negative control. The LUC/REN ratio represents the ability of MdABI5 to activate MdNYC1 and MdNYE1 expression. Asterisks indicate significant differences at *P < 0.05, **P < 0.01