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. 2021 Jul 1;8:159. doi: 10.1038/s41438-021-00593-0

Fig. 4. MdZAT10 accelerates JA-induced leaf senescence.

Fig. 4

a Transcript level of MdZAT10 in response to 100 μM MeJA. b GUS staining and c GUS activity in proMdZAT10::GUS transgenic calli with (MeJA) or without (Mock) 100 µM MeJA treatment for 12 h. d Leaf senescence phenotype and e chlorophyll content of detached leaves from 3-week-old Col and transgenic Arabidopsis MdZAT10-L1, L2, and L3 floated on 3 mM MES buffer with or without 100 μM MeJA for 3 days. f Leaf senescence phenotype and g chlorophyll content of apple leaves transiently expressing empty vector (EV), overexpressing MdZAT10 or MdZAT10 antisense vector floated on 3 mM MES buffer with or without 100 μM MeJA for 14 days. Asterisks indicate significant differences by t-test (*P < 0.05, **P < 0.01)