Fig. 5. MdZAT10 physically interacts with MdBT2.

a, b A Y2H assay showed the interaction between MdZAT10 and MdBT2. Full-length and truncated MdZAT10 and MdBT2 sequences were cloned into the pGBD and pGAD vectors. c In vitro pull-down assay with MdZAT10 and MdBT2. The MdZAT10-His protein was incubated with GST-MdBT2 and GST. Proteins that were pulled down with GST beads were detected using anti-GST and anti-His antibodies. d BiFC assay. The MdZAT10-cYFP and MdBT2-nYFP constructs were transiently expressed in Nicotiana benthamiana leaves, and the fluorescence signal in the nucleus was observed via fluorescence microscopy. Nuclei are indicated by DAPI staining. Scale bars, 10 μm