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. 2021 Jul 1;8:159. doi: 10.1038/s41438-021-00593-0

Fig. 6. MdBT2 mediates MdZAT10 protein stability.

Fig. 6

a Cell-free MdZAT10-His degradation assay. The MdZAT10-His fusion protein was incubated with total protein extracted from wild-type (WT), MdBT2-OX, and MdBT2-Anti transgenic calli for the indicated time periods. b MdBT2 ubiquitinated the MdZAT10-His protein in vivo. MdZAT10-His was immunoprecipitated using an anti-His antibody and then examined using anti-His and anti-Ubi antibodies. IP, immunoprecipitate; IB, immunoblot; Ubi, ubiquitin. c The MdZAT10-GFP protein abundance in transgenic apple calli (MdZAT10-GFP and MdZAT10-GFP/MdBT2-OX) was assessed by immunoblotting using an anti-GFP antibody