Table 1.

Sequences of TP0500, TP0547, TP0574, TP0705, and TP0760 loci that encode the penicillin-binding proteins in TPeC Cz-2020.

Locus*	Protein	Gene position and nucleotide difference relative to TPeC Cuniculi A	Number of SNVs/total gene length [nt]	Protein position and amino acid difference relative to TPeC Cuniculi A
TP0500	Pbp-1	55 T → C	2/1875	19 F → L
		711 T → C		No change
TP0547	LytB	No change	0/1131	No change
TP0574	Carboxypeptidase 47 kDa	No change	0/1305	No change
TP0705	Pbp-2	1,628 A → G	1/2655	543 H → R**
TP0760	Pbp-3	412 C → T	3/1866	138 R → C
		900 A → G		No change
		1,563 G → A		No change

^*Altogether, 8,832 bp were determined, covering the entire sequences of these loci.

^**The amino acid change resulted in a residue that is present among TPA strains.

The determined sequences were compared to those found in TPeC Cuniculi A.