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. 2021 Jun 17;9:687912. doi: 10.3389/fcell.2021.687912

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Copyright © 2021 Li, Zhang, Li, Guo, Li, Zhang, Li and Mi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HnRNPA1 is a powerful sEV-miRNA loading protein. (A) RT-qPCR analysis of mRNA levels after knockdown of hnRNPA1 in A549 cells. (B) Western blotting analysis of protein level in shhnRNPA1 A549 cells. (C) RPM of different small RNA components in sEVs derived from shhnRNPA1 and control (shCTR) cells. (D) Enrichment analysis of the downregulated sEV-miRNAs after knockdown of hnRNPA1. X-axis: 2,585 expressed sEV-miRNA ranked by the expression level. Black lines (enriched in high expression region) represent downregulated sEV-miRNAs and white lines represent upregulated sEV-miRNAs. (E) Percentage of the top 25 highly expressed sEV-miRNAs derived from shCTR and shhnRNPA1 cells. Gray areas represent the top 25 miRNAs, and black areas represent other miRNAs. (F) Bar chart showing the alteration of top 25 highly expressed sEV-miRNAs after knockdown of hnRNPA1. MiRNAs indicated by gray bars were selected for the following RT-qPCR. RT-qPCR analysis of the relative expression levels of the selected miRNAs in shhnRNPA1 sEVs (G) and in shhnRNPA1 cells (H). The significant differences were reported for three independent experiments (ns, no significance; ^∗p < 0.05; ^∗∗p < 0.01, two-tailed t-test). hnRNPA1, heterogeneous nuclear ribonucleoprotein A1; sEV-miRNA, microRNA in small extracellular vesicle; RPM, reads per million.