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. 2021 Jun 17;8:697773. doi: 10.3389/fmolb.2021.697773

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Copyright © 2021 Zheng, Li, Zheng, Fang, Xu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LINC00174 knockdown inhibits the TGF-β/SMAD pathway. (A) The mRNA expression levels of TGF-β1, SMAD2, and SMAD3 in OS cells in sh-LINC00174 or sh-NC group was determined by RT-qPCR analysis. Significance was analyzed by Student’s t-test. (B) Western blot was performed to measure the protein levels of TGF-β1, SMAD2, and SMAD3 in OS cells in sh-LINC00174 or sh-NC group. Significance was analyzed by Student’s t-test. (C) Expression of Smad2 (phospho S255), Smad2 (phospho S467), Smad3 (phospho T179), Smad3 (phospho S213), Smad3 (phospho S423/S425) in MG63 and U2OS cells in the presence and absence of LINC00174 was detected by western blotting. Significance was analyzed by Student’s t-test. *p < 0.05.